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《JOURNAL OF TROPICAL AND SUBTROPICAL BOTANY》 1999-01
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EFFECTS OF MEDIA, GROWTH REGULATORS ANDDIVIDING ON THE GROWTH OF CYMBIDIUM SINENSEPROTOCORMS CULTURED IN VITRO

Chen Li ;Pan Ruichi; Chen Rumin(Biology Department, South China Normal University, Guangzhou 510631)  
The optimal culture conditions of the growth of Cymbidium sinense protocormsinduced from embryo were studied. The results showed that MS was the best basal mediumfor C. sinense protocorm growth and organogenesis. Treatment of 0.5 mg L-1 NAA incombination with 1.0 mg L-1 BA was most satisfactory for protocorm growth. When 1%sucrose and 0.1% activated carbon were added to the basal medium, a superior growth ofprotocorms was observed. Highest increase of the fresh weight of protocorms was at PH 5.0,Protocorms grew well under 15.5 umol m s1 light intensity for 16 hours per day. Combinedtreatment with all optimal single factors mentioned above showed that the fresh weight ofprotocorms was 46% higher than that of the control. As for the increase of proliferationcoefficient, dividing the protocorms by hands gave good result in cutting manner.
【CateGory Index】: Q944.6
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