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《Journal of Hydroecology》 2012-05
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Phylogenetic Analysis and 16S rDNA of Yersinia enterocolitica Isolated from Yellow Catfish(Pelteobagrus fulvidraco)

GOU Xiao-lan1,WANG Li1,HUANG Yan-qing2(1.Key Laboratory of Animal Genetics and Breeding of State Ethnic Affairs Commission & Ministry of Education,College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,P.R.China; 2.East China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences, Shanghai 200090,P.R.China)  
One bacteria strain named GM 2402 was isolated from the heart of natural diseased yellow catfish(Pelteobagrus fulvidraco).The strain was identified according to its morphological characteristics,physiology and biochemistry experiments,as well as,PCR amplification of 16S rDNA genes and sequence analysis.The results showed that strain GM 2402 was a gram-negative bacterium;the ability to movement and MR-VP,salicylic acid,catalase and urease reactions were positive;but L-rhamnose,Galactose,H2S and citrate salt reactions were negative.16S rDNA sequence of strain GM 2402 was 1417 bp and the accession number was JX101598.Strain GM 2402 shared 99.6% identity with Yersinia enterocolitica strains in GenBank database.Phylogenetic tree demonstrated that strain GM 2402 was gathered into one cluster with Y.enterocolitica.Both biochemical character and molecular analysis were proved that strain GM 2402 was Y.enterocolitica.It will benefit the identification of Y.enterocolitica in aquatic animals.
【Fund】: 四川省科技厅应用基础项目(2011JY0095);; 西南民族大学研究生学位点建设项目(2011XWD-S071007)
【CateGory Index】: S943
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