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《Sichuan Journal of Zoology》 2008-03
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Inhibitory of Acetylcholinesterase in Silkworm by Natural Product Extracts

HUANG Xiao,TENG Yun,TAO Ke,HE Yan,GENG Yu-cong,HOU Tai-ping(College of Life Science,Sichuan University,Chengdu 610064)  
Acetylcholinesterase(AChE)was purified from the brain tissues of silkworm by Sephadex G-25,DEAE-Sepharose Fast Flow and Sephadex G-200 chromatography.Organophosphorus pesticide was used as positive control to compare the inhibitory effects on crude and purified AChE.The result indicated that the purified AChE is pure after the purification procedures,the inhibition of purified AChE was more sensitive than that of crude AChE.In addition,extracts from natural products have higher inhibition on the purified AchE than the cruded.The IC50 of purified AChE by alcohol extract of spiraea is 0.091×10-3 g·mL-1.
【Fund】: 国家自然科学基金(20372052)
【CateGory Index】: S482.3
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