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《Journal of Sichuan University(Natural Science Edition)》 2008-01
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Purification and characterization of acetylcholinesterase,a kind of pesticide target

PENG Xia,TAO Ke,TENG Yun,HUANG Xiao,HOU Tai-Ping(Key Laboratory of Bio-resources and Eco-environment of Ministry of Education,College of Life Sciences,Sichuan University,Chengdu 610064,China)  
Acetylcholinesterase(AChE) of Bombyx mori Linaeus,separated from the crude extract,was purified to electrophoretic homogeneity by Sephadex G-25 chromatography,DEAE-Sepharose Fast Flow ion-exchange chromatography and Sephacryl S-200 gel filtration,respectively.The Molecular weight of the purified enzyme was 77.8 kDa,measured by SDS-PAGE.The optimum temperature of the AChE was 37 ℃,above which the enzyme would be unstable.It exhibited optimum activity at pH 7.5.The Michaelis constant for acetylthiocholine iodide was 0.392 mmol/L.The optimum concentration of substrate was 1.6 mmol/L,and the enzyme could be inhibitied by high level of acetylthiocholine iodide.
【Fund】: 国家自然科学基金(20572076);; 教育部博士点基金(20050610016)
【CateGory Index】: TQ450
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【References】
China Proceedings of conference Full-text Database 1 Hits
1 WANG Xia,LIU Ming-jun,GAO Jun-e,CHEN Jun-wu,LIU Shun-qin,LI Dun ( College of Natural Resources and Environment,South China Agricutural University,Guangzhou 510642,China);Advances in Purification and Utilization of AChE from Aquatic Animals[A];[C];2009
【Citations】
Chinese Journal Full-text Database 6 Hits
1 ZHAO Jian-Zhuang et al(Beijing Agricultural College ,Beijing 102206);Study on Parition and Purification of Acetylcholinesterase[J];Journal of Beijing Agricultural College;2003-04
2 PENG Yu, WANG Yin Chang, HAN Zhao Jun, CHEN Chang Kun, LI Guo Qing (College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China);Distribution and purification of acetylcholinesterase in Chilo suppressalis[J];Acta Entomologica Sinica;2002-02
3 Gao Xiwu Zhou Xuguo Wang Rongjing Zheng Bingzong (Department of Entomology, China Agricultural University, Beijing 100094);DISTRIBUTION AND PURIFICATION OF ACETYLCHOLINESTERASE IN COTTON BOLLWORM (LEPIDOPTERA:NOCTUIDAE)[J];ACTA ENTOMOLOGICA SINICA;1998-S1
4 ZHANG Ying~(1,2), HUANG!Jian~1, GAO Xi-wu~(2*)(1. College of Plant Protection, Fujian Agricultural and Forestry University, Fuzhou 350002, China; 2. Department of Entomology, China Agricultural University, Beijing 100094, China);Comparison of the Head Acetylcholinesterase Sensitivity to Insecticides between Two Honeybee Populations[J];Chinese Journal of Pesticide Science;2005-03
5 Ding Shihua & Li Qingyi(Dept Fisheries,SW Agricul Univ,Chongqing 630716)(Dept life Sci,SW Norm Univ,Chongqing 630715);PURIFICATION AND CHARACTERIZATION OF CHOLINESTERASE FROM EISENIA FOETIDA[J];CHINESE JOURNAL OF APPLIED AND ENVIRONMENTAL BIOLOGY;1997-03
6 HE Yi-guo,ZHANG Ke-jun,XIAO Bo,HOU Tai-ping(College of Life Science,Sichuan University,Chengdu 610064,China);Study on the Antifeedant Activity and Mechanism for Bioactive Compound from Stellera chamaejasme against Larvae of Pieris rapae[J];Chinese Journal of Biological Control;2006-S1
【Co-references】
Chinese Journal Full-text Database 5 Hits
1 JIANG Shuang~1,ZHANG Ying-ge~(1*),ZHANG Sa~2(1.Institute of Pharmocology and Toxicology,Academy of Military Medical Sciences,Academy of Military Medical Sciences, Coordination Laboratory of National Center for Nanoscience and Technology,Beijing 100850;2.National Center of Biomedical Analysis,Beijing 100850,China);AFM observation of the effects of acetylcholine and PAS inhibitor on the structure of tetrameric acetylcholinesterase[J];Journal of Chinese Electron Microscopy Society;2009-03
2 ZHANG Qiu ju\+1, CHENG Xiao hua\+1, WEI Zhao rong\+1, MA Jie\+2, TIAN Ying\+2, WU Bing\+2, SHAO Jia xing\+3, LIU Biao\+1 (1.Xinjiang Sanitation and Antiepidemic Station, Urumqi Xinjiang 830011, China; 2.Antiepidemic Station of Urumqi Railway Bureau, U;Investigation of Agricultural Chemical Pollution Level in Xinjiang Underground Water[J];Arid Environmental Monitoring;2001-03
3 LI Yongyu, HONG Huasheng, WANG Xinhong *, HONG Liyu, YE Cuixing State Key Laboratory of Marine Environmental Science, Environmental Science Research Center, Xiamen University, Xiamen 361005,China;Estimation of the sources of organophosphorus pesticides in Xiamen sea area[J];Acta Scientiae Circumstantiae;2005-08
4 WEI Hui1,2, SHEN Jin-liang1, WU Wei1, ZHAO Jian-wei1, ZHAN Zhi-xiong1 (1.Institute of Plant Protection, Fujian Academy of Agricultural Sciences, Fuzhou 350013,China; 2.College of Plant Protection, Nanjing A- gricultural University, Nanjing 210095,China);Purification,Biochemical Properties and Insecticides Susceptibility of Acetylcholinerase from Housefly(Musca domestica L.)[J];Journal of Agro-Environment Science;2009-01
5 ZHU Xiao-shan~(1),MENG FAN-ping~(2),HE Dong-hai~(2)(1.College of Environmental Science and Engineering,Nankai University,Tianjin 300071,China;2.College of Marine Life Science,Ocean University of China,Qingdao 266003,China);Purification and Partial Biochemical Characterization of Acetylcholinesterase from Scomberomorus Niphonius(Cuvier)[J];Journal of Qingdao University(Engineering & Technology Edition);2006-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Zewen et al (Nanjing Agric Univ,Nanjing,Jiangsu 210095);Susceptibilities of Brown Planthopper and White Backed Planthopper from Anqing[J];Journal of Anhui Agricultural Sciences;2002-04
2 REN Xiao-Xia HAN Zhao-Jun WANG Yin-Chang (Key Lab of Monitoring and Management of Plant Disease and Insects, Ministry of Agriculture,Nanjing Agricultural University, Nanjing 210095, China);CLONING AND SEQUENCE ANALYSIS OF A cDNA FRAGMENT ENCODING ACETYLCHOLINESTERASE IN COTTON BOLLWORM (HELICOVERPA ARMIGERA)[J];Acta Zoologica Sinica;2002-01
3 YANG Jianmei, MIAO Jianqiang, (Department of Biology, Peking University);Studies on the comparison of AChE properties between the Apis sinensis Smith and Apis mellifera Linnaeus[J];Acta Scicentiarum Naturalum Universitis Pekinesis;1992-06
4 HAN Zhao-jiu,HAN Zhao-jun 1 (Key Laboratory of Monitoring and Management of Plant Diseases and Pests,Ministry of Agriculture, Nanjing Agricultural University,Nanjing 210095,China);Cloning and Sequence of Nicotinic Acetylcholine Receptor α Subunit from Chilo suppressalis[J];Zoological Research;2002-01
5 LI Fei,HAN Zhao-jun 1 (Key Laboratory of Monitoring and Management of Plant Diseases and Pests,Ministry of Agricultural, Nanjing Agricultural University,Nanjing 210095,China);Cloning and Sequencing of Two Acetylcholinesterase cDNA Fragments from Cotton Aphid,Aphis gossypii Glover[J];Zoological Research;2002-05
6 Huang Yan et al.(Guangzhou Medical College, Guangzhou 510182);Enzyme Ticket and Chromophoric Substrate Ticket for the Convenient and Rapid Detection of Organophosphorus Pesticides[J];CHINESE JOURNAL OF ENVIROMENTAL SCIENCE;1995-03
7 Yang Le′en(Dept. of Plant Protection,Zhongkai Agr.Technique College,Guangzhou,510225);EFFECT OF HOST PLANTS ON THE SUSECPTIBILITY OF THE GREEN PEACH APHID Myzus persicae(Sulzer)TO INSECTICIDES[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1995-01
8 LIAO Xiao-Lan 1,ZHU Shui-Fang 2,LUO Kuan 1 (1.College of Plant Protection,HNAU,Changsha 410128,PRC;2.Institute of Animal and Plant Quarantine,Beijing 100029,PRC);Up-Date Development of Molecular Detecting Technology on Gene Mutation and Single Nucleotide Polymorphism[J];Journal of Hunan Agricultural University;2002-05
9 SUNHongwu, 2 HANZhaojun, 2 WANGYinchang, 1BAILixin ( 1 Instituteof Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing2 10 0 14;2 Departmentof Plant Protection,Nanjing Agricul tural University,Nanjing 2 10 0 95 );Organophosphorus Insecticide Resistance in Cotton Bollworm( Heli- coverpa armigera H bner) in Major Cotton Cultivated Areasof Jiangsu Province[J];Jiangsu Journal of Agricultural Sciences;1999-04
10 Zhang Han Xing Zhi-yong Sun Man-chi(Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences);POLYACRYLAMIDE GEL ELECTROPHORESIS OF ACETYLCHOLINESTERASE[J];Bulletin of the Academy of Military Medical Sciences;1983-06
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