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《Journal of Shandong Agricultural University》 2001-02
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DNA TEMPLATE MAKING AND TECHNOLOGY SYSTEM BUILDING FOR AFLP ANALYSIS OF APPLE GENOME

WANG Cai-hong 1,WANG Qian 2,DAI Hong-yi 3,JIA Jian-hang 2,WANG Bin 2,SHU Huai-rui 1 (1 College of Horticulture,Shandong Agricultural University,Taian 271018,China; 2 Genetic Institute of Chinese Academy of Science,Beijing 100101 3 Laiyang Agri  
In general,AFLP analysis demands high quality DNA If apple genomic DNA was extracted by general method (SDS),it should be purified probably In the system of apple AFLP analysis,the template density is an important factor which affects the result of selective amplification Then more attention should be paid to primers combination and denature condition of selective amplification products before electrophoresis
【Key Words】： apple DNA template AFLP analysis
【CateGory Index】： Q943.2
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