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《Journal of Shandong Agricultural University(Natural Science Edition)》 2009-02
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MA Yong-fei,QU Xiang-jin,YIN Huan-shun,DU Hong-xia(College of Chemistry and Material Science,Shandong Agricultural University,Taian 271018,China)  
The L-tryptophan molecular imprinting film sensor,which select 2-mercaptobenzimidazole and L-tryptophan as monomer and template molecule,respectively,was prepared directly on the surface of gold electrode by cyclic voltammetry method.Because of L-tryptophan has no electrical activity,the potassium ferricyanide was used as a molecular probe to detect L-tryptophan by constant potential amperometry.The response achieve stable in 100 s.The sensor exhibited good selectivity and sensitivity to L-tryptophan.The linear range for the determination of L-tryptophan is from 3.10×10-5 to 8.83×10-4 mol/L,the binding constant of 2-mercaptobenzimidazole and L-tryptophan were detected,and the best detection condition of this sensor were discussed.
【Fund】: 国家自然科学基金项目(No.20775044);; 山东省自然科学基金项目(No.Y2006B20)
【CateGory Index】: TP212.2
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