Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Fisheries of China》 2011-11
Add to Favorite Get Latest Update

Characteristics of quenching enzyme AiiO-AIO6 and its effect on Aeromonas hydrophila virulence factors expression

ZHANG Mei-chao1,CAO Ya-nan1,YAO Bin1,BAI Dong-qing2,ZHOU Zhi-gang1(1.Key Laboratory for Feed Biotechnology of the Ministry of Agriculture,Feed Research Institute, Chinese Academy of Agricultural Sciences,Beijing 100081,China; 2.Department of Fisheries Science,Tianjin Agricultural College,Tianjin 300384,China)  
The prokaryotic expression vector pET28a/aiiO-AIO6 was constructed by inserting the aiiO-AIO6 gene into expression vector pET28a and transformed into E.coli.Subsequently,the recombinants with activity of N-acyl homoserine lactonase were investigated.After purification with nickel column,enzymatic characteristic of the target protein was researched.Moreover,the effects of purified enzyme solution on the expression of Hemolysin,Cytotonic enterotoxin,Extracellular protease,Serine protease,Phospholipase A1,ea tl five virulence factors from Aeromonas hydrophila ATCC7966 were detected by real-time quantitative PCR.The purified AiiO-AIO6 showed that the N-acyl-homoserine lactonase had optimal pH and temperature at pH 8.0 and 30 ℃,respectively.The enzyme was stable between pH 6.0 and 11.0,retained over 65% enzyme activity at 80 ℃ for 30 min.It resisted various neutral proteases and chemical reagents.The fusion protein can hydrolyze many N-acyl homoserine lactones substrates.With N-(3-oxo-octanoyl)-L-homoserine lactone as substrate,the Km value of AiiO-AIO6 was 0.015 mmol/L and specific activity was 583.33 U/mg.The expression amount of five virulence factors of A.hydrophila had down-regulated trend obviously(P0.05)at 8 h and 12 h.with adding the purified enzyme liquid for the expression amount of five virulence factors In this study the optimal pH and temperature of AiiO-AIO6 were obtained by detecting enzymatic property,and AiiO-AIO6 could inhibit A.hydrophila virulence factors expression indirectly by degrading signal molecules,which established a theoretical foundation for the application in aquaculture environment and biological control of pathogenic A.hydrophila.
【Fund】: 科技部农业科技成果转化资金项目(2010GB23260591);; 中国农业科学院饲料研究所所长基金(2009);; 中国博士后科学基金(20090450472)
【CateGory Index】: S941
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 5 Hits
1 CHEN Tao~(1***) QIAN Gou-liang~(1***) YANG Xiao-li~2 MA Jun-yi~2 HU Bai-shi~(1**) LIU Feng-quan~1(1.Key Laboratory of Monitoring and Management of Plant Diseases and Insects,Ministry of Agriculture,Department of Plant Pathology,Nanjing Agricultural University,Nanjing 210095,China;2.Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China);Detection of Quorum Sensing Signal Molecule of Acidovorax avenae subsp,citrulli and Its Regulation on Pathogenicity[J];Journal of Agricultural Biotechnology;2008-04
2 WU Qian,YAN Fang,LIU Feng-bo (Shanxi Agricultural University,Taigu,030801;Chain);Research Progresss on Aeromonas hydrophila[J];Livestock and Poultry Industry;2010-02
3 WU Huai-guang, YE Wei-xing, YU Zi-niu, SUN Ming (State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology,Huazhong Agricultural University, Wuhan 430070);High Efficient and Stable Expression of AiiA Protein in Bacillus thuringiensis[J];Scientia Agricultura Sinica;2007-10
4 QIU Jian,LI Cheng-guang,JIA Zhen-hua,ZHANG Xia,MA Hong,SONG Shui-shan(Biology Institute of Hebei,Shijiazhuang 050051,China);Enzymatic characterization and function of N-acylhomoserine lactonase SS10[J];Acta Phytopathologica Sinica;2007-06
5 Meichao Zhang,Yanan Cao,Bin Yao,Zhigang Zhou* Key Laboratory for Feed Biotechnology of the Ministry of Agriculture,Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China;High expression and characterization of N-acyl-homoserine lactonase-xylanase recombinant protein[J];Acta Microbiologica Sinica;2011-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 CHEN Wan-guang et al(Department ofLife Science,LuoyangTeachers College,Luoyang,Henan 471022);Study on the Diagnosis and Control Methods of Japanese Kio Diseases[J];Journal of Anhui Agricultural Sciences;2007-23
2 XU Da-yong et al(Department of Animal Science,Xichang College,Xichang,Sichuan 615013);The Therapeutic Effect of Onion on the Pathogenic Bacteria of Gill Rot Disease in Grass Carp[J];Journal of Anhui Agricultural Sciences;2008-15
3 HUANG Yuan-yuan et al(Biology Institute of Hebei,Shijiazhuang,Hebei 050081);Effects of C_6-HSL on Potato Growth and Disease Resistance Ability[J];Journal of Anhui Agricultural Sciences;2010-02
4 HUANG Yu-liu et al (Fishery Institute of Guangxi, Nanning, Guangxi 530021);Application of Chinese Herbal Medicine in the Health Raise of Aquiculture[J];Journal of Anhui Agricultural Sciences;2010-11
5 DU Ming - shan (Anyang University,Anyang 455000,China);The Present Developing Situation of Green Aquatic Fodder Additives and the Future Prospect[J];Journal of Anyang University;2003-02
6 He Shunlian,(Department of Fisheries, Hunan Agricultural University, Changsha, 410128);AN ACUTE TOXIC EFFECT OF MIECHONGLING,SHACHONGWEI,JUNDUWEI ON WHITMANIA PIGRA[J];Journal of Beijing Fisheries;2001-04
7 WANG Wen-bin,TANG Lin,LI Jian-zhong,ZENG Bo-ping,LUO Yu-shuang,HAN Qing (Key Laboratory of Zoology in Hunan Higher Education,College of Life Sciences,Hunan University of Arts and Science,Changde 415000,China);A preliminary investigation on the infection of Monopterus albus by Trypanosoma monopteri in West Dongting Lake Basin[J];Journal of Hunan University of Arts and Science(Natural Science Edition);2010-02
8 WU Huixian1,XUE Junzeng2(1College of Life Science,Zhejiang University,Hangzhou 310012,China; 2Department of Biology,Hangzhou Normal College,Hangzhou 310012,China);Preliminary Study on the Dominant Bacteries in the Soft-shelled Turtles Ponds[J];Fisheries Science;2003-02
9 WEN Ji wen 1,FU Ze-tian 1,LI Dao liang 1,XING Ke zhi 2,CAI Zhong hua 2 (1 The Engineering Institution of China Agriculture University,Beijing,100083,China) (2 The Aquaculture Department of Tianjin Agriculture Institution,Beijing,300384,China);Model of Fish Disease Diagnosis[J];Fisheries Science;2003-02
10 SONG Wenhua1,FU Lijing1,WANG Lei1,YU Xiang Cui1,Shuangfa2 (1Freshwater Fisheries Research institute of Liaoning province,Liaoyang 111000,China; 2Dahuofang Reservoir Administrative Bureau,Fushun 113000,China);Acute Toxicity of Three Kinds of Medicines to Pelteobagrus fulvidraco[J];Fisheries Science;2003-05
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 GUO Chuang,WANG Yongkun(College of Animal Husbandry and Veterinary Medicine,Yangzhou Univers ity,Yangzhou 225009,China);Advancement in Study on Aeromonas hydrophila[J];Fisheries Science;2003-06
2 LV Hui-yuan1,2 ZHOU Zhi-gang2* Florence Rudeaux3 Frédérique Respondek3(1.College of Animal Science,Jiangxi Agriculture University,Nanchang 330045,China;2.Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3.Beghin Meiji,Thumeries 59239,France);Effects of Dietary Short Chain Fructo Oligosaccharides on Intestinal Microflora,Mortality and Growth Performance of Oreochromis aureus ♂×O.niloticus♀[J];Chinese Journal of Animal Nutrition;2007-06
3 HUANG Tian-pei~1,YANG Mei~2,YAO Fan~1,HUANG Zhang-min~1,YU Xiao-min~1,HUANG Zhi-peng~1,HUANG Bi-wang~1[1.Key Laboratory of Biopesticide and Chemical Biology(Fujian Agriculture and Forestry University),Ministry of Education,Fuzhou,Fujian 350002,China;2.College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350007,China];Cloning and fusion expression of aiiA gene from Bacillus cereus[J];;2006-03
4 DONG Chuan-fu, LIN Tian-long (Fish Disease Research Center of Animal Husbandry and Veterinary Medicine Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China);Research progress on Aeromonas hydrophila[J];Fujian Journal of Agricultural Sciences;2003-04
5 LI Nan1,2,SUN Yi-min1,2,TIAN Li-ying1,LI Xiao-yue2,SHEN Hong-qi3,WANG Feng-min3,LI Quan-zhen3,CAO Jie-ying3,ZHAO Bao-hua2 (1.Department of Pharmaceutical Engineering,Hebei Chemical Engineering and Pharmaceutical College,Hebei Shijiazhuang 050026,China;2.College of Life Science,Hebei Normal University,Hebei Shijiazhuang 050016,China;3.Department of Diseases Supervision,Hebei Aquatic Product Office,Hebei Shijiazhuang 050011,China);The Isolation and Identification of Aeromonas hydrophila[J];Journal of Hebei Normal University(Natural Science Edition);2009-02
6 Wu Huimin1,2 Lin Wenhui2 Shi Cunbin2(1.Dalian Fisheries University, Dalian 116023, China;2.Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China);Research Process on Aeromonas hydrophila[J];Hebei Fisheries;2007-03
7 Wen Xiaofang1,2 Huang Junsheng1,2 (1 Environment And Plant Protection Research Institute, CATAS, Danzhou, Hainan, 571737;2 State Key Lab for Tropical Crops Biotechnology, CATAS, Haikou, Hainan, 571101);Quorum-Sensing of Bacteria and Its Signal Molecules[J];Journal of South China University of Tropical Agriculture;2005-01
8 Chen Changfu;Shi Weizhou;Zhao Guizhen;Li Mingzhen(Fishery College,Huazhong Agricultural University, Wuhan 430070);ISOLATION AND IDENTIFICATION OF PATHOGENIC BACTERIA CAUSING ROTED GILL DISEASE IN MANDARINFISH(SINIPERCA CHUATSI BASILEWSKY)[J];JOURNAL HUAZHONG(CENTRAL CHINA) AGRICULTURAL UNIVERSITY;1995-03
9 LI Yue-hong1,ZHANG Pei-jun2,GUO Wei-sheng1,CUI Ying-ying1,WANG Xin1(1.Jilin Agriculture University,Changchun Jilin 130118;2.The Center of Examination and Monitor in Jilin Province,Chan-gchun Jilin 130000);Isolation and Identification of Aeromonas Hydrophila[J];Jilin Animal Husbandry and Veterinary Medicine;2007-11
10 ZHENG Guang-yu(College of Life Sciences, Beijing Normal University, Beijing 100875 );Research Progress on Eucariotic Expression System[J];Journal of Kashgar Teachers College;2004-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved