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Studies on the Detection of Brucella Antibody of Pig Using Dot PPA ELISA

CAO San jie, WEN Xin tian, LI Ji xiang (SiChuan Agriculture Univesity, Yaan 625014, China)  
A Dot PPA ELISA is developed for the pig serum antibodies detection of Brucellosis and SAT of Brucellosis controlled in this studies. The results indicate that Dot PPA ELISA is a very sensitive and specific test, the minimum amount of pig sera IgG detected by using Dot PPA ELISA ammounts to 6 992×10 -9 g and its specificity is perfect in crossreaction tests with the positive pig serum of PRV, PPV, HCV, JEV, CHL et al. Many advantages of Dot PPA ELISA to detect the Brucella antibodies are described: the test is easy and rapid, no special instrument needed, its results are easy to be determined and the reaction membrain can be stored for a long time et al. In a word, Dot PPA ELISA can be uesed widely to detect the Brucella antibodies of pig and has great significance to detect Brucella antibodies quickly.
【CateGory Index】: S854.4+3;S858.285.
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