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《Journal of Sichuan University of Science & Engineering(Natural Science Edition)》 2011-05
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Optimization of PCR-DGGE Electrophoresis Conditions of Eucaryotic Microorganisms Community in Daqu

LUO Hui-bo,HOU Hai-bo,HUANG Zhi-guo,WEI Chun-hui,YE Guang-bin(Liquor-making Biotechno logy & Application key Laboratory of Sichuan province,Sichuan University ofScience & Engineering,Zigong 643000,China)  
DGGE was applied to eukaryotic microbes community of Daqu in the experiment.DGGE electrophoresis conditions for 18S rDNA of eukaryotic microbes were optimized as follows: Gel concentration was 8%,denatured range was between 40% ~ 70%,electrophoresis ran 4 minutes at first as the voltage was 200V and then ran for 14 hours as the voltage was 130V.Under these conditions,satisfactory separation results could be achieved by electrophoresis.
【Fund】: 四川省科技厅应用基础项目(07JY029-026;2008JY0091);; 四川省教育厅重大培育项目(07ZZ018);; 四川理工学院引进人才项目(2007-18)
【CateGory Index】: TS261.11
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