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《JOURNAL OF SICHUAN NORMAL UNIVERSITY(NATURAL SCIENCE)》 1999-06
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Study on Enzymolysis of Jerusalem Artichoke and Its Comprehensive Utilization

LUO Ying 1 LI Jun gang 2 ( 1. Department of Biology, Mianyang Teacher’s College, Mianyang 621000, Sichuan; 2. Mianyang Research Center of Biotechnology, Mianyang 621000, Sichuan)  
Jerusalem artichoke is rich in Inulin . Directly decomposig Inulin with Inulalse can produce high fructose syrup. This article mainly introduces the research achievement, comprehensive utilization of fermented products, preparation of Inulalse , high fructose syrup and alcohol by using microbial fermentation process and pointed out the prospect of fermented products in food industry. A mutant AL 154 Inulase activity reaches 146.3 μ/mL. The suitable conditions of enzymolysis are as follows: Jerusalem artichoke extracted juice with 10%~20% of the total suger as the substrate, 3.0 units (the activity unit using Inulin as substrate) of enzymatic activity used pergram total sugar of the substrate, pH5.0, temperature 60℃, with enzymolysis for 10h. Under these conditions, the highest hydrolytic rate of the substrate is 97.2%. The hydrolysate containes about 86.1% fructose.
【Fund】: 四川省教委青年科研基金
【CateGory Index】: TQ92
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