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《Sichuan Journal of Physiological Sciences》 2004-01
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Role of Secretory Factors and Live Bacteria of Klebsiella pneumoniae Induced IL-8 Expression in Human Lung Epithelial Cells

Chen Xiangwen, Wang Fang, Zhou Minyan, Feng Yun, Huang Ning, Wang Boyao, Wu Qi (Research Institute of Infection and Immunity,West China Medical Center,Sichuan University,Chengdu 610041)  
Objective: To investigate the in vitro effects of K. pneumoniae and its secretory factors on the expression of IL 8 in human lung epithelial cells. Methods:Bacterial conditioned media and live bacteria of two K. pneumoniae clinical isolates were prepared. Human lung epithelial cell lines A549 and SPC A 1 were challenged with the conditioned media and the K. pneumonia respectively; and the expression level of IL 8 was detected using ELISA.Results: ① The dose dependent increased expression of IL 8 was observed after the two cell lines were challenged with the bacterial conditioned media from two K. pneumoniae isolates for 24 hours (P0.01). ② A549 was infected with live bacteria of two K. pneumoniae isolates or E. coli DH5α respectively for 2 hours, and before a second 24h incubation period with fresh medium the extracellular bacteria were killed with the addition of gentamicin. K. pneumoniae significantly induced the production of IL 8 by A549 cells as compared with DH5( that only manifested moderate effect on the expression of IL 8 (P0.01). [WT5”HZ]Conclusion:[WT5”BZ] Secretory factors from K. pneumoniae and intracellular K. pneumoniae could up regulate the expression of IL 8 in human lung epithelial cells, suggesting that airway epithelial cells play an important role in the process of the host inflammation responses to K. pneumoniae.
【Fund】: 国家自然科学基金 (No .30 2 70 6 88);; CMB基金(No.98 86 1)资助
【CateGory Index】: R392
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