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《Sichuan Medical Journal》 2004-09
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An research on the expression of IL-8 in PMVEC directly-induced by LPS and its mechanism through the Nuclear Factor-Kappa B

Gu Dayong 1,Wang Hua 1,Liu Jinbiao 1,et al.1.Moleuclar Biology Center,Research Institute of Surgery,The Third Military Medical University,Chongqing 400000,China;2.Clinical Experimental Institute,Chengdu Army General Hospital,Chengdu 610083,China  
Objective To study IL-8 expression of pulmonary microvascular endothelial cells (PMVEC)directly induced by LPS and its mechanism through the Nuclear Factor-Kappa B.Methods When PMVEC is directly excited by 100ng/ml LPS at 0h,0.5h,1h,2h,4h,6h,8h or 1ng/ml,10mg/ml,100ng/ml LPS at 1h or 6h,the expressions of IL-8mRNA in PMVEC and its protein secretion in the supernatant of culture medium were examined by the methods of ISH,ELISA respectively and the activation of NF-κ Β was detected by EMSA.And those were then to be compared with which was inhibited by PDTC.Results LPS could directly act on the PMVEC to promote the IL-8 expression,including promoting the expression of IL-8mRNA and secretion of IL-8 protein,and the mRNA expressions increased before the protein secreted in terms of time.The LPS excitement could also significantly promote the activation of NF-κ Β, which peaked at 1h and then went down.PDTC could significantly decrease the expression of IL-8 and the activation of NF-κ Β.Conclusion The directly-induction of LPS could exactly promote the activation of NF-κ Β,then cause to the expression and secretion of IL-8.SO as to provide the required physical condition for the PMN migration.It could be an important role in inflammatory response induced by LPS.
【CateGory Index】: R563.8
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