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《Journal of Shandong University(Natural Science)》 2005-03
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The factors affected transformation efficiency of Lactococcus lactis by electroporation

SUN Lei,KONG Wen-tao & KONG Jian *(State Key Laboratory of Microbial Technology, Shandong Univ.,Jinan 250100, Shandong, China)  
Electrotransformation factors were performed to transfer plasmid pTRKH_2 into Lactococcus lactis MG1614. Cell growth phase, electric field strength, resistance and incubation time all affect transformation efficiency. The optimum factors are the cell harvested at the middle exponential growth phase, electroporation at 10.0kV/cm and 400Ω, 2h cell incubation time. The transformation efficiency goes up to 1.2×10 3 transformants per μg DNA with those optimum factors, which is higher than optimiz those reported before.
【Fund】： 山东省中青年科学家奖励基金资助项目(01BS12)
【CateGory Index】： Q933
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