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《Journal of Shandong University(Natural Science)》 2007-05
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Purification and characterization of a new kind of antimicrobial peptide by fungi-penicillium sp.M03

CHEN Yu-juan1,JI Ai-guo1,2,* and GAO Pei-ji3(1.School of Pharmaceutical Sciences,Shandong Univ.,Jinan 250012, Shandong,China;2.International Biotechnology Research & Development Center,Shandong University at Weihai,Weihai 264209,Shandong,China;3.State Key Lab of Microbial Technology,Shandong Univ.,Jinan 250100,Shandong,China)  
A kind of antimicrobial peptide AF2 was purified from the broth of fungi.Some properties of AF2 were studied.The broth was subjected to filtration,centrifugation and ultra-filtration.After being freeze-dried,the crude AF2 product was purified by Sephadex LH-20 molecular sieve chromatography,DEAE-Sepharose FF and Sephadex G-15,and a pure component AF2 was obtained.The results show that AF2 has the characteristic reactions of peptides,whose molecular weight is about 1155.The data analysis of amino acids show that AF2 contains twelve kinds of amino acids,Asp,Glu,Ser,Gly,Arg,Thr,Ala,Pro,Cys,Ile,Leu and Phe.AF2 is stable to β-lactamase,acid and alkali,which heat-resistance is also well.
【Fund】: 国家自然科学基金资助项目(30600026 30270047)
【CateGory Index】: Q936
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