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《JOURNAL OF SHANDONG UNIVERSITY(NATURAL SCIENCE EDITION)》 1999-02
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TRANSFORMATION OF S.CEREVISIAE BY ELECTROPORATION

Qin Yujing,Jin Jianling,Bao Xiaoming,Gao Dong (State Key Lab.of Microbiol.Technol.,Shandong Univ.,Jinan)  
Electrotransformation was performed to transfer shuttle plasmid pYES2 into S.cerevisiae .Voltage、cell grown、incubation time and plasmid concentration affect transformation efficiency.The best conditions are the cell harvested at the exponential growth phase、5 kV、0.7 μg plasmid DNA and 1 h incubation time,transformation efficiency is up to 4.8×10 5 transformants per μg plasmid DNA,which is higher than intact cell transformation.The cell viability is about 39.2%.
【Fund】: 山东省自然科学基金
【CateGory Index】: TS261.11
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1 Qin Yujing ;Liu Shili; Bao Xiaoming; Gao Dong(Dept. of Microbiology Shandong University, Jinan);High efficiency transformation of intact yeast cell[J];BIOTECHNOLOGY;1997-03
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