Determination of Cholesterol in Edible Oil by Gel Permeation Chromatography-High Performance Liquid Chromatography
CHEN Guo;Chongqing Institute for Food and Drug Control;
Objective To establish the method for determination of cholesterol in edible oil by high performance liquid chromatography(HPLC) coupled with gel permeation chromatography(GPC). Methods Edible oil was dissolved in ethyl acetate-cyclohexane and then purified by GPC with a flow rate 5.0 m L/min. The portions between 18-24 min were collected then separated on a C18 column and detected by a diode array detector in 205 nm. Results The linear range of cholesterol was 0.05-0.8 mg/m L, the limit of detection was 7.07 mg/kg. The recoveries were from 69.96 % to 93.87 %, and the relative standard deviations(RSDs) were from 0.38 % to 6.76 %. Conclusion The pretreatment of this method is easy-operating and high automatic, thus it is suitable for detection of cholesterol in edible oil.
【CateGory Index】： O657.72;TS227