The extraction of carotenoids from Rhodotorula
(Food & Biotechnology Department, Zhengzhou Institute of Light Industry, Zhengzhou 450002)
The methods of breaking cell walls and extracting carotenoids were studied. The experimental results showed that the method of combining hydrochloric acid (HCL) with heating (by boiling water) was suitable for the cell rupturing from Rhodorula RY - 98, and its optimal conditions were as follows: HCL concentration 2 mol/L, HCL addition 12 ml/g (dry cells), soaking time 40 min and heating time 4 min. Using acetone of 20 mL/g (dry cells) was the most extracting method. Under the above optimal conditions, the yield of carotenoids could reached 450.2 μg/g (dry cells).