Effectiveness of activation of sigma-1 receptor on reducing myocardium injury in ischemia reperfusion
LIAO Xiaoping;YU Pujiao;XU Jiahong;Department of Cardiology, Anting Hospital,Shanghai;Department of Cardiology, Tongji Hospital Affiliated to Tongji University;
[Objective] This paper attempts to investigate whether sigma-1 receptor(Sig1 R) activation attenuate myocardial ischemia reperfusion injury(MIRI) and whether Sig1 R inhibition exacerbates MIRI in mice model. [Methods] C57 BL/6 mice are injected via tail vein with BD1047(Sig1 R inhibitor), SA4503(Sig1 R activator) or saline3 days before MIRI surgery. Then, double-staining technique and western blot analysis are performed on myocardium tissue. [Results] Mice that have received BD1047 pretreatment demonstrate a significant increase in the myocardial infarction size after I/R compared with control group(P 0.05), and a significantly increased expression of Bax and Caspases-3 proteins and a significantly reduced expression of Bcl-2 protein compared with sham control, MIRI control and sham BD1047 groups(P 0.05). Mice that have received SA4503 pretreatment demonstrate a significant reduction in myocardial infarction size after I/R compared with control group(P 0.05), and a significantly reduced expression of Bax and cleaved Caspases-3 proteins and a significantly increased expression of Bcl-2 protein compared with sham control, MIRI control and sham SA4503 groups(P 0.05).[Conclusions] SA4503 protects MIRI and reduces myocardial infarction whereas BD1047 deteriorates MIRI and augment myocardial infarction, and these effects may be achieved through activation or inhibition of Sig1 R.