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《Journal of Shandong Univenity(Health Scicnes)》 2004-06
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Protective effects of gypenoside against oxidative neurotoxicity induced by glutamate

XIN Hua, SHANG Lin-shan, LIU Jin-Cheng, et al(Institute of Cell Biology, School of Medicine, Shandong University, Jinan 250012, Shandong, China)  
Objective: To study the protective effects of gypenoside(GP) against oxidative neurotoxicity induced by glutamate. Methods: Primary cortical neurons were prepared from embryonic day 13.5~14 Wistar rats. The oxidative neurotoxicity model was obtained by applying high dosage glutamate. The viability of cells after various treatments was determined by using the method of 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide reduction. The death style of neurons was assayed through transmission electron microscope, and flow cytometry was applied to check the difference of cell apoptosis ratios. Results: GP significantly attenuated glutamate-induced neurotoxicity in primary cultured cortical neurons with a time-dependent manner. The optimal cell viability was obtained by a pre-treatment with GP. Through transmission electron microscope we found that oxidative stress exerted by glutamate induced a form of programmed cell death with characteristics of both apoptosis and necrosis in primary cultured neurons. Flow cytometry analysis showed the apoptosis ratio of glutamate-intoxicated cells (21.63%)was apparently higher than those in both untreated control (0.09%) and the GP-treated cells(8.94%). Conclusion: These results suggest that GP exerts significant neuroprotective effects on cultured cortical cells. Therefore, GP may be efficacious in protecting neurons from oxidative damage produced by exposure to excess glutamate.
【Fund】: 山东省卫生厅中医药局资助课题(1999)。
【CateGory Index】: R285
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Chinese Journal Full-text Database 2 Hits
1 HAN Yu-xia1,WEI Xin-bing1,LI Xia2,DING Yan1,XIN Hua2,DING Hua1 (1.Institute of Pharmacology,School of Medicine,Shangdong University,Jinan 250012,China;2.Institute of Cell Biology,School of Medicine,Shangdong University,Jinan 250012,China);Protective effects of gypenosides against glutamate-induced hippocampus injury in rats[J];Journal of Shandong University(Health Sciences);2008-05
2 SUN Tao,ZHANG Dao-lai,XIE Shan-shan,WANG Yu-zhuo,FENG Yu-xin,XIN Hua(Institute of Cell Biology,School of Medicine,Shandong University,Jinan 250012,China);Effect of ethanol on expression of connexin 43 in primary culture of neural precursor cells[J];Journal of Shandong University(Health Sciences);2009-06
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