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《Journal of Shandong University(Health Sciences)》 2006-06
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Protection of gypenoside on glutamate-induced oxidation in the cultured cortical neurons of embryonic rats

WANG Xu-ping,ZHAO Ling,FENG Yu-xin,SHANG Lin-shan,LIU Jin-cheng,CAO Wei-peng,ZHU Xiao-yin,XIN Hua(Institute of Cell Biology,School of Medicine,Shandong University,Jinan 250012,Shandong,China)  
Objective: To explore the neuroprotective effects of gypenoside(GP) on glutamate-induced cytotoxicity to cortical neurons and investigate its signaling pathway.Methods: The oxidative neurotoxicity model was constructed in primary cortical neurons isolated from Wistar rat E13~14 embryos following a high dose glutamate to the neurons directly.GP was added before the damage.The viability of cells after various treatments was detected by MTT method.Ho33342 and PI double labeled method was used to analyse cellular death pathways.The contents of NO and H_2O_2 were detected.The changes of mitochondria membrane potential of neurons were analyzed by flow cytometry.Results: Glutamate triggered two cellular death pathways: necrosis and apoptosis.The cells,exposed toglutamate(2 mmol/L for 12hours),showed charateristic change of damage,which could be relieved by GP (200 μg/ml) with cell survival rate increment.GP could block the increase of NO and H_2O_2,and the decrease of mitochondria memrane potential induced by glutamate.Consequently,the survival rate of neurons was greatly increased.Conclusion: Gypenoside can prevent the neurons from the damage of glutamate oxidative neurotoxicity and raise the neuron survival rate,which may be due to cleaning of the free radicals and improving of the mitochondrial function.
【Fund】: 山东省卫生厅中医药局科研基金资助课题(1999)
【CateGory Index】: R285
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