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《Journal of Shandong University(Health Sciences)》 2006-07
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Establishment of NSCLC in situ metastasis model using GFP/A549 cells

WANG Ya-wei~1,WANG Xiu-wen~1,WEI Jun-min~1,LIU Chun-xi~2(1.Department of Oncology;2.Key Laboratory of Cardiovascular Remondeling and Function Research,ChineseMinistry of Education and Chinese Ministry of Public Health,Qilu Hospital of Shandong University,Jinan 250012,Shandong,China)  
Objective: To construct the nude mouse model of human non-small cell lung cancer(NSCLC) metastasis by using GFP/A549 cells.Methods: The vector pRNAT-U6.1/Neo was transfected into A549 cells and selected the stabilizing GFP cells with G418,and then the in vivo and in vitro activity of GFP/A549 and A549 cells were compared.Nude mice were performed orthotopic transplantation of GFP/A549 cells and killed when achieved the reserved standard.Pathological and immunohistochemistry analysis were used to detect the metastasis,and KODAK IS2000MM was used to check the dissemination and the metastasis of tumor.Results: Stable clones expressing GFP were isolated,and there were no significant difference between in vivo and in vitro growth of two cell lines(P0.05).The NSCLC metastasis model was established via orthotopic transplantation.The levels of CEA and LRP of primary tumors and metastases determined by LEICA IM50 had no significant difference(P0.05).Pathological and immunohistochemistry examinations discovered brain metastases were found in 11 mice,liver metastases in 4,whereas KODAK IS2000MM showed 9 and 4,respectively.Both had no significant difference((P0.05).) Conclusion: GFP labeled A549 cells orthotopic transplantation can create NSCLC metastasis model more easily than transitional methods.KODAK IS2000MM could be used to monitor tumor metastasis and dissemination non-invasively.
【Fund】: 山东省科技厅科技计划资助课题(2004GG3202017)
【CateGory Index】: R734.2
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【References】
Chinese Journal Full-text Database 1 Hits
1 CHEN Fang-lin CHEN Zheng-tang (Cancer Institute of PLA,Xinqiao Hospital,Third Military Medical University,Chong qing 400037,China);Establishment of a lung cancer cell line marked by green fluorescent protein[J];Chongqing Medicine;2007-19
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 DU Ming,LI Shao-lin,LU Fa-jin,et al.(1.Department of Cardiothoracic Surgery,the First Affliliated Hospital,Chongqing University of Medical Sciences,Chongqing 400016,China;2.Teaching and Research Section,Chongqing University of Medical Sciences,Chongqing 400016,China;3.Department of Oncology,the Third People's Hospital,Chongqing 400014,China);The pilot study on metastasis model of highly metastasic human large cell lung cancer cell lines in nude mice[J];Chongqing Medical Journal;2005-08
2 ZHANC Pei-Tong; PRO Bing-Kui; PEI Ying-Xia et al.(Flow Cytometry laboratory, Guanganmen Hospital , China Academy of Traditional Chinese Medicine, Beijing 100053);Measurement of the antigens immunologically related to the platelet glycoproteinson tumor cells surface by now cytometry[J];CHINESE JOURNAL OF IMMUNOLOGY;1999-12
3 Cun Wang,Zong-Guang Zhou,Zhao Wang,Yang-Chun Zheng,Gao-Ping Zhao,Department of General Surgery,West China Hospital,Sichuan University,Chengdu 610041,Sichuan Province,China Dai-Yun Chen,Department of Pathology,West China Hospital,Sichuan University,Chengdu 610041,Sichuan Province,China;Nodal spread and micrometastasis within mesorectum[J];世界胃肠病学杂志(英文版);2005-23
【Secondary References】
Chinese Journal Full-text Database 2 Hits
1 ZHANG Xin-sheng1,LIU Xiao-yu1,REN Shuang-yi2(1.Seven-Year Program of Clinical Medicine,Dalian Medical University,Dalian 116044,China;2.Department of General Surgery,the Second Affiliated Hospital of Dalian Medical University,Dalian 116027,China);Discovery and application of green fluorescent protein[J];Journal of Dalian Medical University;2009-04
2 Shuzhen WEI~*,Yong SONG~*,Zhijian YANG~Δ*Department of Respiratory Medicine,Nanjing General Hospital of Nanjing Command,Nanjing University,Nanjing 210002,China;ΔNanjing Origin Center for Tumor Animal Model,Nanjing 210002,China;Recent Advances on in vivo Imaging with Fluorescent Proteins for the Research of Lung Cancer[J];Chinese Journal of Lung Cancer;2009-03
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