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《Journal of Shandong University(Health Sciences)》 2008-04
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Protective effect of gypenosides-containing serum against oxidation neurotoxicity induced by glutamate

LIU Lu-hua,LU Su-mei,SUN Tao,ZHANG Dao-lai,FENG Yu-xin,XIN Hua(Institute of Cell Biology,School of Medicine,Shandong University,Jinan 250012,China)  
Objective To investigate the neuroprotective effects of gypenosides (GP) containing serum on glutamate(Glu) induced oxidation toxicity to cortical neurons.Methods Sera containing GP was collected by way of seropharmacology,and the primary cortical neurons isolated from Wistar rats E14-15d embryos were studied.The viability of cells was detected by the MTT method,the cellular death pathway was analyzed by Ho33342 and the PI double-labeled method,the contents of SOD and GSH were determined by a biochemistry method,and Cytosolic [Ca2+] was determined by flow cytometry(FCM).Results Compared with Glu,GP-containing serum could increase the cell survival rate and block the decrease of SOD and GSH and the increase of cytosolic.Conclusions GPcontaining serum can protect the neurons from damage of glutamate oxidative neurotoxicity and the 400 mg/kg/d GP-containing serum has the strongest antagonistic action.
【Fund】: 山东省自然科学基金资助项目(Y2006C41)
【CateGory Index】: R285
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