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Determination of Quercetin in the Extract of Bluish Dogbane by High—performance Lquid Chromtography

Yan Fu-sheng  
In this Paper, a high performance liquid chromatographic method for the determina tion of quercetin in the extract of bluish dogbane was reported.A Shimadzu liquid chromatograph Lc—2 with a column (25cm×4.6mm) of YWG*CH (C18chemicaliy bonded stationary phase) was used.50%methanol containig 1.5% phosphoric acid was chosen as the mobile phase.Detect ion was at 254nm,The quantity of quercetin was determined by internal standard method with β-naphthol as a reference.The result showed that the mean content of quercetin in bluish dogbane was 0.17Bwith a standard deviation of 3.2%(eight determinations).The proposed method is simple, rapid and reproducible.
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