Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Shandong Medical Journal》 2009-31
Add to Favorite Get Latest Update

Study on the bioactivities of Group A rotavirus VP7 gene expression product

WU Jing-hua,WANG Hai-xin,ZHAO Jie,DONG Ai-ying(Affiliated Hospital of North China Coal Medical University,Tangshan 063000,P.R.China)  
Objective To induce and analyze the cloned Group A rotavirus VP7 gene expression and its bioactivities,so as to lay a foundation for the construction of genetic engineering vaccine against rotavirus. Methods The VP7 gene fragment was amplified and purified,then combined with pEDM27/5 and transformed into lacotobacillus .After induced by lactose,the VP7 protein was combined specifically with mouse monoclonal antibody,and the bioactivities of which was determined. Results The VP7 gene was amplified and transformed into pEDM27/5 successfully. DNA(+) recombinant was selected by blue and white plague,and the lacotobacillus were transformed ,a protein chalaza of about 28 kD was found by SDS-PAGE after the induction of lactose,and which could be combined specifically with the VP7 protein against rotavirus. Conclusions VP7 protein of restructuring lacotobacillus has good bioactivities;this can lay a foundation for the further development of genetic engineering vaccine against rotavirus.
【Fund】: 唐山市科技局课题项目(08130227C)
【CateGory Index】: R392
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 LI Jin tao, WU Yu zhang, FEI Lei, ZOU Li yun, TANG Yan, MOU Zhi rong , GENG Miao (Institute of Immunology, PLA, Third Military Medical University, Chongqing 4000 38, China);Cloning and expressing of human rotavirus vp7 in potatoes[J];Immunological Journal;2003-01
2 Jia Shifang\ Wang Yinyu\ Guo Xinhua\ Huan Liandong (Institute of Microbiology,The Chinese Academy of Sciences,Beijing 100080);The Factors Affected Transformation Efficiency of Lactobacillus by Electroporation[J];CHINESE JOURNAL OF BIOTECHNOLOGY;1998-04
3 Ren Zhiqiang 1,3 \ Liu Huimin 2\ Li Runzhi 3\ Yang Huizhen 1 (1 Institute of Crop Genetics Shanxi Academy of Agricultural Sciences Taiyuan\ 030031\ 2 Shanxi Academy of Agricultural Sciences Taiyuan\ 030031\ 3 Shanxi Agricultural University\ Taigu\ 030801);The Application of Transgenic Plants as Bioreactor in Vaccine[J];Progress In Biotechnology;2003-06
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Er-qin~(1,2),LI Shi-fang~(2),WANG Chun-feng~(1),QIAN Ai-dong~(1) (1.Animal Science and Medicine College of Jilin Agricultural University ,Changchun,Jilin,130118,China; 2.Institute of Plant Protection, China Academy of Agriculture Science, Beijing,100094,China);Progress on Transgenic Plant Rotavirus Vaccine[J];Progress In Veterinary Medicine;2005-08
2 HUO Dan-qun~(1), FAN Shou-cheng~(1,2), ZHANG Yun-ru~(1), HOU Chang-jun~(1), FAN Shou-jun~(2) gineering Chongqing University, Ministry of Education, Chongqing University, Chongqing 400030, China; 2.Chongqing Pig Breeding Science Research Institute,Fan Shoucheng, Chongqing 402460, China);Cloning of aspergillus niger F246 phytase gene and its a new type of expression vector construction[J];Journal of Chongqing University(Natural Science Edition);2004-08
3 Zhu Huiying Wu Cunxiang Hu Baozhong Li Wenbin Han Tianfu Institute of Crop Science, The Chinese Academy of Agricultural Sciences, Beijing, 100081 College of Life Science, Northeast Agricultural University, Harbin, 150030;Progress in Plant-derived Oral Vaccine Production[J];Molecular Plant Breeding;2004-03
4 Yu Liyun, Zhao Zhigang, Hou Xilin, Cao Hongwei, Wang Guihua (Heilongjiang August First Land Reclamation University, Daqing 163319);Study on the Surface Displayed Porcine Epidemic Diarrhea Viral(PEDV) S1 on Lactic Acid Bacteria[J];Journal of Heilongjiang August First Land Reclamation University;2008-04
5 LI Jin-tao, FEI Lei , MU Zhi-rong, TANG Yan, WEI Jing, WU Yu-zhang (Institute of Immunology, Third Military Medical University, Chongqing 400038, China);Study on immune responses in Balb/c mice by oral immunization with rotavirus antigen expressed in transgenic potatoes[J];Immunological Journal;2005-06
6 HUANG Yong,ZHANG De-chun* (Department of Clinical Microbiology, Chongqing University of Medical Science, Chongqing 410016, China);Cloning and Expression of the Manganese Superoxide Dismutase Gene of E. coli in Lactobacillus bulgaricus[J];Food Science;2005-05
7 GAO Xu-wen1,2,CHEN Hui3,JIA Rui-qing2,YAO Li-hong2,MA Chang-wei1,ZHANG Zhi-qing2, (1.College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2.Institute for Virus Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China; 3.Capital Medical University, Beijing 100069, China);Expression and Bioactivities Study on IFN-IgG Fc Fusion Protein in Lactobacillus casei[J];Food Science;2007-01
8 XIA Zi-fang,SHI Gui-yang,ZHANG Liang,CHEN Wei,WANG Zheng-xiang (Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China);Optimization of Electroporation Processing of Lactobacilli[J];Food Science;2008-02
9 KANG Jie-fang,WANG Zhe-zhi (College of Life Sciences,Shaanxi Normal University,Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry,Xi'an,Shaanxi 710062,China;Progress in Transgenic Plants Producing Pharmaceutical Proteins[J];Progress in Modern Biomedicine;2006-09
10 Bao Hui-fang1 Chen Chuang-fu2 Chen Fu-shuang3 Ren Xue-yan1 Wang Yan-qin4(1.Laboratory of Xinjiang Endemic and Ethnic Diseases Shihezi University-Bioengineering College of Shihezi University,Shihezi Xinjiang 832003;2.Laboratory of Xinjiang Endemic and Ethnic Diseases of Shihezi University-Animal Technology College of Shihezi University,Shihezi Xinjiang 832003;3.Vinisuntime Internation Co.Ltd,Yili Xinjiang 835215;4.Bio-engineering College of Shihezi University,Shihezi Xinjiang 832003);The prensent Situation and Prospect of Transgenic Plant as oral Vaccine[J];Animal Science and Veterinary Medicine;2004-11
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 QIN Yun long 1,JIN Ning yi 2,LUO Kun 1,LI Fan 1,WANG Hong wei 2, WANG Li xin 2, ZHANG Yong sheng 1 , YIN Zhen 2 (1.Department of Microbiology, Norman Bethune University of Medical Sciences,Changchun 130021, China; 2 Viral Laborat;Construction of DNA vaccine with HIV-1 gag and hIL-2/hIL-6 and its immunological study[J];Immunological Journal;2001-01
2 MAO Xu hu, LU Dong shui, GUO Hong,WU Chao,WANG Fu kun,ZOU Quan ming (Department of Clinical Microbiology,Third Military Medical University, Chongqing 400038,Chian);Recombination and expression of a component of urease B subunit transmembrane protein of helicobacter pylori[J];Immunological Journal;2001-02
3 Yang Guofeng Zhou Peng Zhang Chunfa Zheng Xueqin (National Key Biotechnology Laboratory for Tropical Crops,South China Academy of Tropical Crops,Haikou 571101);Progresses on Transgenic Plant-derived Vaccines[J];Journal of Agricultural Biotechnology;2001-03
4 FENG Zhen (College of Forestry,Shangdong Agricultural University,Taian 271018,Shandong,China);Current Status of Edible Vaccines[J];Life Science Research;2001-S1
5 Geng De-gui Wang Yi-qin Li Wen-bin Sun Yong-ru(Institute of Genetics,Chinese Academy of Sciences,Beijing 100101,China);The current situation of oral vaccines produced in transgenic plants[J];Progress In Biotechnology;2002-01
6 Zhu Guo-ping Xu Chong(Laboratory of Genetic Engineering,College of Life Sciences,University of Science and Technology of China,Hefei 230026);Progress in Biotechnology of Green Vaccines[J];Progress in Biotechnology;2002-02
7 SONG Chang zheng 1,Hugh S Mason 2 (1 Shandong Medical Biotechnology Center,Shandong Academy of Medical Sciences,Jinan 250062; 2 Boyce Thompson Institute,Cornell University,NY 14853);Expression hemagglutinin vaccine of avian influenza virus in transgenic potato[J];Biotechnology;2001-05
8 YANG Guofeng1 ZHOU Peng1 WANG Jianwei2 1 National Key Biotechnology Laboratory for Tropical Crops China Academy of Tropical Agriculture Science Haikou 571101 2 Institute of Virology China Academy of Preventive Medicine Beijing 100052;Progresses and Prospects of Studies on Rotavirus Vaccine[J];Biotechnology Information;2001-01
9 Li Yinu 1 Hu Yingkao 2 Shen Guifang 1 ( 1 Biotechnology Research Institute Chinese Academy of Agricultural Sciences, Beijing 100081; 2 Biology Department Capital Normal University, Beijing 100037);Genetic Engineering Vaccines in Transgenic Plants[J];Biotechnology Information;2002-02
10 Liu Dehu Biotechnology Research Center, CAAS, Beijing 100081;Plant as a System for Production of Pharmaceutical Proteins[J];BIOTECHNOLOGY INFORMATION;1999-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved