Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Shandong Medical Journal》 2011-11
Add to Favorite Get Latest Update

Effects of Gas on Aβ secretion in APP695 and 293 cells which overexpressed APP

LIU He-ping1,CHENG Cong,WANG Zhao,FAN Li(1 Anhui Medical University,Hefei 230032,P.R.China)  
Objective To investigate the possibility of gastrodine(Gas) to treat Alzheimer′s disease(AD) and its principle.Methods N2a cells(APP695) and 293 cells(293SW) were divided into two groups respectively.The experimental group was treated with Gas of 25,50,100,200 μM,and the control group was treated with normal medium.Morphology was observed via inverted microscope,cell vitality was tested via MTT assay,and Aβ level was detected through ELISA assay.Results Compared with control group,there were no significance in morphology,axonal retraction,bad attachment,cell suspension or cluster.No significant differences were observed in the survival rate of Gas treated cells.But Aβ level was greatly reduced in a dose-dependent manner.Conclusion Gas could be a potential compound for AD treatment,which may result from the depressing of Aβ expression.
【Fund】: 国家重点基础研究发展计划(973计划)资助项目(2007CB507406);; 清华—裕元医学科学研究基金(THYY20070008)
【CateGory Index】: R285
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 LIU Zhong-hua, HU Hai-tao, FENG Gai-feng, ZHAO Zhi-ying, MAO Nai-ying. Department of Human Anatomy, Medical School of Xi'an Jiaotong University, Xi'an 710061, China;Protective Effects of Gastrodin on the Cellular Model of Alzheimer's Disease Induced by Aβ25-35[J];Journal of Sichuan University (Medical Sciences Edition);2005-04
2 Xue Liuhua , Tang Yipeng, Hong Qingtao et al.(Beijing University of Traditional Chinese Medicine, Beijing 100029);Protective Effects of Gastrodine on Injured Cortical Neurons by Glutamic Acid[J];JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE;1999-01
3 YANG Pan,SONG Qiu-jie,WANG Yue-hua,et al.The Department of Pharmacology,Xuanwu Hospital of Capital Medical University,Key Laboratory for Neurodegenerative Diseases of Ministry of Education,Beijing 100053,China;Effects of Epimedium Flavanoids on β-amyloid in APP695 cDNA-transfected Nerve Cells and on β-secretase Activity in Vitro[J];Chinese Journal of Rehabilitation Theory and Practice;2009-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YANG Yi, TANG Rui(School of Science, Beijing University of Chemical and Tech Chemistry & Chemical Biology,Ministry of Education;Department of Chemistry, Tsinghua University, Beijing 100084);Characteristics of macroporous adsorption resin in adsorption and separation on gastrodin in gastrodia extract[J];Analytical Laboratory;2004-11
2 LIU Zhong-hua, HU Hai-tao, FENG Gai-feng, ZHAO Zhi-ying, MAO Nai-ying. Department of Human Anatomy, Medical School of Xi'an Jiaotong University, Xi'an 710061, China;Protective Effects of Gastrodin on the Cellular Model of Alzheimer's Disease Induced by Aβ25-35[J];Journal of Sichuan University (Medical Sciences Edition);2005-04
3 WU Zhong-Liang①,REN Ning②(①Department of Neurology,②Department of Comprehensive Diagnosis and Therapeutic Center,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,Shaanxi China);Effect of Gastrodin Injection on the Neuronal Apoptosis of the Brain After Focal Cerebral Ischemia Reperfusion[J];Pharmaceutical Journal of Chinese People's Liberation Army;2008-03
4 LI Yi, XING Xuan, ZHAO Lun, LOU Shu-Jie, HE Cheng, LU Chang-Lin (Department of Neurobiology,Second Military Medical University,Shanghai 200433,China);Differentiation of mesencephalic progenitors induced by different chemicals in vitro[J];Chinese Journal of Anatomy;2004-04
5 Song Ruifeng , Tang Yipeng , Hong Qingtao (Beijing University of Traditional Chinese Medicine, Beijing 100029);Dynamic Changes in bcl-2 and bax Expressions in Hippocampal Astrocytes in the Mice Undergone Transient Forebrain Ischemia and Reperfusion: the Effects of Kangdai No.1[J];Journal of Beijing University of Traditional Chinese Medicine;2002-04
6 NIU Pi-ye 1 ,NIUQiao 1 ,HE Shu-chang 2 (1.Department of Occupational Health,School of Public Health,Shanxi Medical University,Taiyuan030001,China;2.Medical Department,Beijing University);Effects of Gastrodia Elata on the Alteration of Amino Acid in the Brain Cortex Induced by Aluminum in Rats[J];Journal of Labour Medicine;2004-01
7 Li Miaoling,et al Department of Myocardial Electrophysiology,Luzhou Medical College;THE CULTURE METHOD OF NEWBORN RAT CEREBRAL NEURONS AND ITS BASIC ELECTROPHYSIPLOGICAL PROPERTIES[J];Journal of Luzhou Medical College;2003-05
8 ;天麻素对颅脑损伤模型脑保护作用的实验研究[J];China Foreign Medical Treatment;2009-35
9 DONG Zhuan-nian,GONG Xiu-hui,PENG Bo,LIU Xiao-zhu(Southwest Foresty College,Kunming 650224,China);Research of Purification the Peptides from Gastrodiae elata B1.[J];Biotechnology;2007-06
10 ;The clinical application and fundamental pharmacological study on gastrodin[J];Acta Academiae Medicinae CPAPF;2007-04
【Secondary Citations】
Chinese Journal Full-text Database 7 Hits
1 Yang Zhong;Cai Wenqin(Department of Histology and Embryology,Third,Military Medical University,Chongqing,630038);Taurine minimizes excitatory amino acid-induced neurotoxicity on cultured neonatal rat cerebellar cortical neurons[J];ACTA ACADEMIAE MEDICINAE MILITARIS TERTIAE;1996-01
2 Zhong Yuguo;Pang Qijie;Zhang Henpyu;Tao Aiqun;Zhang Zuqian;Gao Mingyue;Song Yinglan Department of Pharmaceutical Chemistry Department of Pharmacology;SYNTHESIS AND ANTICONVULSIVE ACTIVITY OF GASTRODIGENIN HOMOLOGUES AND ANALOGUES[J];;1984-01
3 Xue Liuhua , Tang Yipeng, Hong Qingtao et al.(Beijing University of Traditional Chinese Medicine, Beijing 100029);Protective Effects of Gastrodine on Injured Cortical Neurons by Glutamic Acid[J];JOURNAL OF BEIJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE;1999-01
4 Hong Qingtao; Tang Yipeng(Department of Pathology. Beijing University of Traditional Chinese Medicine, Beijing);NEURONS OF CEREBRAL CORTEX OF NEWBORN RATS IN PRIMARY CULTURE[J];CHINESE JOURNAL OF NEUROANATOMY;1994-03
5 ZHAO Bin~1,YANG Hong-jun~2,ZHAO Jing~1(1.The Medical Laboratory of Kunming Military General Hospital,Kunming 650032,China; 2.The First Affiliated Hospital of Kunming Medical College,Kunming 650031,China);A screen assay of β-secretase inhibitors in vitro[J];Chinese Pharmacological Bulletin;2006-01
6 M Liu and JT Zhang(Department of Pharmacology,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050);PROTECTIVE EFFECTS OF GINSENOSIDE Rb1 AND Rg1 ON CULTURED HIPPOCAMPAL NEURONS[J];ACTA PHARMACEUTICA SINICA;1995-09
7 Mu Jingyu; Xong Wen; Yang SbiJie; et al.(Department of Biochemistry, Basical Medical college of Norman Bethune University of Medical Sciences, Changchun 130021);Free radical mediates glutamate neurotoxicity in primary cultures of rat cortical neurons[J];JOURNAL OF APOPLEXY AND NERVOUS DISEASES;1996-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved