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Effects of propolis flavonoids pinobanksin-3-acetate on expression of PDE4D and Gadd45G gene in human colon cancer cell line

Chimengul Turghun;Buhediche Ababekri;Zulpiye Ismayil;Meryemgul Erkin;Adalet Memet;Yimit Rahman;College of Life Science and Technology,Xinjiang University;  
Objective To investigate the inhibitory effect of propolis flavonoids pinobanksin-3-acetate( PB3A) on the human colon cancer cell line SW480 and to explore the underlying mechanism. Methods The cultivated human colon cancer cell line SW480 was randomly divided into the PB3A group and the control group. Cells in the PB3A group were intervened by 100 μg / mL PB3A,and the control group without intervention. We observed the cellular morphology by invert microscope after incubation for 24 h of the two groups. Real-time fluorescence quantitative polymerase chain reaction and Western blotting were performed to measure the expression of phosphodiesterase 4D( PDE4D) and growth arrest and DNA damage-inducible gene G( Gadd 45G) at mRNA and protein levels. Results After treated with 100 μg / mL PB3A,the colon cancer cells became shrunk,smaller,refraction decreased with granular material appeared intra-celluler in the PB3A group. In comparison with those in the control group,the mRNA and protein expression of PDE4D was down-regulated, while the mRNA and protein expression of Gadd45G was up-regulated( all P 0. 01). Conclusions PB3A inhibited the proliferation of SW480 cells and induced the apoptosis. Its possible mechanisms may be related to down-regulation of PDE4D expression and up-regulation of Gadd45G expression.
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