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《Shandong Medical Journal》 2018-47
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Effects of AFP gene silencing on migration and adhesion of hepatocellular carcinoma cells

WANG Li;HE Tao;FENG Shijie;WAN Baishun;WANG Xiaoqian;ZHANG Min;ZHANG Ling;HAN Feng;Henan Tumor Hospital Affiliated to Zhengzhou University;  
Objective To observe the effects of AFP gene silencing on migration and adhesion of hepatocellular carcinoma cell line EGHC-9901 and its mechanism. Methods EGHC-9901 cells were divided into three groups: the experimental group which was transfected with AFP-siRNA,the vector control group which was transfected with empty vector,and the blank control group without treatment. Transwell assay was applied to detect cell migration ability; the cell aggregation assay was applied to evaluate alterations of homogeneous adhesion ability whereas Laminin adhesion assay was used to assess its heterogeneous adhesion ability; Western blotting was used to detect the expression of migration and adhesion-related proteins E-cadherin,CD44 V6,CD15,integrin β5,β-catenin,and α-catenin. Results Transwell assay demonstrated more cells of the experimental group( 132 ± 16) that migrated through the polycarbonate membrane than those of the vector control group( 335 ± 21) and blank control group( 371 ± 32)( P 0. 05). Cell aggregation assay witnessed markedly higher aggregation index of the experimental group( 0. 530 ± 0. 020 and 0. 632 ± 0. 031),versus that of the vector control group( 0. 223 ± 0. 017 and 0. 322 ± 0. 021) and the blank cotrol group( 0. 240 ± 0. 012 and 0. 376 ± 0. 019) when the incubation time was 40 min and 60 min,with significant difference( all P 0. 05). When we cultured cells for 60 min,the OD values of the experimental group,vector control group and blank control group were 0. 157 ± 0. 008,0. 347 ± 0. 026,and 0. 363 ± 0. 039,respectively,with statistically significant difference( all P 0. 05). At 90 min,the OD values of the experimental group,the vector control group and the blank control group were 0. 139 ± 0. 010,0. 476 ± 0. 023,and 0. 503± 0. 045,respectively,with statistically significant difference( all P 0. 05). The expression levels of integrin β1 protein in the experimental group,vector control group and blank control group were 0. 337,0. 078,and 0. 051,respectively; the expression levels of CD15 protein in the experimental group,vector control group and blank control group were 0. 221,0. 629,and 0. 631,respectively; significant difference was found between the experimental group,vector control group and blank control group( all P 0. 05). Conclusions AFP gene silencing inhibits the cell migration,promotes homogeneous adhesion and inhibits heterogeneous adhesion by regulating the integrin β1 and CD15 expression.
【Fund】: 河南省基础与前沿研究项目(082300450110)
【CateGory Index】: R735.7
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 HE Tao,ZHANG Ling,CUI Hong,WANG Yun-jian,HUANG Chang-shan,HUANG Tao,HAN Feng Department of Hepatopancreatobiliary Surgery,Henan Tumor Hospital,Zhengzhou 450008,Henan Province,China;Effect of stable and transient transfection with RNAi on expression of AFP in hepatocellular carcinoma cell line EGHC-9901[J];军医进修学院学报;2010-11
2 QI Tong-gang, WANG Yun-shan, WANG Fang, et al (Dept. of Molecular Biology, The Second Hospital of Shandong University, Jinan 250000, China) [;Construction and identification of a plasmid expressing SiRNAs in mammalian cells aimed at AFP gene[J];山东大学学报(医学版);2004-03
3 WU Xiaopeng, WANG Zhanmin, LIU Bo, LIU Jun, GAO Yingmao, LI Zhaoting, LIU Chunsheng. Department of General Surgery, Qilu Hospital,Shandong University, Ji′nan 250012, China;Establishment and characterization of human extrahepatic growing hepatocellular carcinoma cell line EGHC-9901[J];中华外科杂志;2002-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Nian FANG;Ziling FANG;Huiqing ZHANG;Xiaoni HAN;Gen HUANG;Nongrong WANG;Jianping XIONG;Kunhe ZHANG;Gastrointestinal Department of Internal Medicine, The Fourth Affiliated Hospital of Nanchang University;The First Affiliated Hospital of Nanchang University;The Third Department of Medical Oncology, Jiangxi Provincial Tumor Hospital;Department of Gynecology and Obstetrics, The Fourth Affiliated Hospital of Nanchang University;;Survivin mRNA is suppressed in HepG2 cells after AFP gene silencing[J];中国肿瘤临床;2013-24
2 ZHANG Weihua1,GUAN Shiyun2,FAN Ruiqin2 1.Department of Internal Medicine,the Second People's Hospital of Songzi in Hubei Province,Songzi 434217;2.The Second People's Hospital of Jingzhou,China;Report of pedunculated hepatocellular carcinoma in 2 cases[J];胃肠病学和肝病学杂志;2013-03
3 ZHANG Tao1,BAI Fang-yun1,BAI Fei-hu2(1.Ningxia Medical University,Yinchuan 750004,China;2.the Affiliated Hospital of Ningxia Medical University,Yinchuan 750004,China);Screening of HO-1 gene silencing stable transfection cell lines of gastric cancer cell line SGC7901[J];东南大学学报(医学版);2012-05
4 LU Liyou ZHAO Fuling HE Changhong YANG Hongmei JIANG Hong Yantaishan Hospital,Yantai 264000;siRNA's repressing AFP to voice male stomach cancer cell FU97 is vs AFP the proliferation represses and apoptosis[J];滨州医学院学报;2011-06
5 RAN YUN,DENG XIN,YANG DA-GUO,et al.1.Hubei University of Traditional Chinese Medicine(Wuhan Hubei,430061)China;Establishment of HepG2 cell line lost expression of the alpha fetoprotein[J];中西医结合肝病杂志;2011-05
6 HE Tao,CUI Hong,WANG Yun-jian,HUANG Chang-shan,HUANG Tao,HAN Feng,ZHANG Ling(Dept.of Hepatopancreatobiliary Surgery,Henan Tumor Hospital,Zhengzhou 450008,China);Effects of AFP silencing on proliferation of hepatocellular carcinoma cell line EGHC-9901[J];基础医学与临床;2011-08
7 ZHANG Ling,HE Tao,CUI Hong,WANG Yunjian,HUANG Changshan,HUANG Tao,HAN Feng Department of Hepatopancreatobiliary Surgery,Henan Tumor Hospital,Zhengzhou 450008,China;Effects of AFP gene silenced by siRNA on apoptosis of hepatocellular carcinoma cell line EGHC-9901[J];胃肠病学和肝病学杂志;2011-01
8 HE Tao,ZHANG Ling,CUI Hong,WANG Yun-jian,HUANG Chang-shan,HUANG Tao,HAN Feng Department of Hepatopancreatobiliary Surgery,Henan Tumor Hospital,Zhengzhou 450008,Henan Province,China;Effect of stable and transient transfection with RNAi on expression of AFP in hepatocellular carcinoma cell line EGHC-9901[J];军医进修学院学报;2010-11
9 WU Hai-jiang,DONG Chao,CUI Ning,LUO Yong,CAO ChiXuzhou Central Hospital of Jiangsu,Xuzhou 221009,R.R.China;Diagnosis and interventional treatment of extrahepatic growing hepatocellular carcinoma[J];医学影像学杂志;2006-10
10 SHI Yang, LI Xiang-nong, LI Wen-mei, LU Kui-yang, SHEN Shi-gang (Department of General Surgery,The Affiliated Hospital of Xuzhou Medical College, Xuzhou,Jiangsu 221002,China);Clinical features and surgical treatment of extrahepatic growing hepatocellular carcinoma[J];中国普通外科杂志;2003-09
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 LI Gao-feng1,WANG Hong-mei2,CHEN Long-hua2 1Department of Oncology,Liuzhou Workers Hospital,Liuzhou 510515,China;2Department of Radiation Oncology,Nanfang Hospital,Southern Medical University,Guangdong 510515,China;Effect of ATM gene silence on growth of HepG2 cells[J];军医进修学院学报;2009-04
2 Yun-Shan Wang, Xiao-Li Ma, Central Laboratory, Jinan Central Hospital, Clinical Medical College of Shandong University, Jinan 250013, Shandong Province, China Tong-Gang Qi, Yue-Sheng Meng, Guang-Ju Guan, Laboratory of Clinical Molecular Biology, The Second Hospital of Shandong University, Jinan 250015, Shandong Province, China Xiang-Dong Liu, Shandong Provincial Hospital, Jinan 250020, Shandong Province, China;Downregulation of alpha-fetoprotein siRNA inhibits proliferation of SMMC-7721 cells[J];World Journal of Gastroenterology;2005-38
3 QI Tong-gang, WANG Yun-shan, WANG Fang, et al (Dept. of Molecular Biology, The Second Hospital of Shandong University, Jinan 250000, China) [;Construction and identification of a plasmid expressing SiRNAs in mammalian cells aimed at AFP gene[J];山东大学学报(医学版);2004-03
4 WU Xiaopeng, WANG Zhanmin, LIU Bo, LIU Jun, GAO Yingmao, LI Zhaoting, LIU Chunsheng. Department of General Surgery, Qilu Hospital,Shandong University, Ji′nan 250012, China;Establishment and characterization of human extrahepatic growing hepatocellular carcinoma cell line EGHC-9901[J];中华外科杂志;2002-08
5 Hu Chuanmin,Liu Yanfang,Sui Yanfang,Xu Liqing,Liu Chenggang Department of Pathology ,Xi’an 710033;Establishment of a human hepatocelldar carcinoma cell line HCC-9204 and its characteristics[J];第四军医大学学报;1995-02
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