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《Shandong Medical Journal》 2018-47
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Expression of APOBEC3s-like cytidine deaminase gene family members in patients with hepatocellular carcinoma

CHEN Qingmei;CHEN Rui;DENG Yang;The Second Hospital of Shandong University;  
Objective To investigate the expression and prognostic significance of apolipoprotein B mRNA-editing enzyme catalytic polypeptide 3( APOBEC3 s)-like cytidine deaminase gene family members in hepatocellular carcinoma( HCC). Methods Real-time quantitative PCR and immunohistochemistry were used to detect the mRNA and protein expression levels of APOBEC3 s-like cytidine deaminase gene family members in tumor tissues and adjacent normal tissues from 155 HCC patients. Results The relative mRNA expression levels of A3 A,A3 B,A3 C,A3 D,A3 F,A3 G,and A3 H in the tumor tissues of HCC patients were 4. 08 ± 0. 11,6. 61 ± 0. 17,6. 42 ± 0. 10,5. 29 ± 0. 12,6. 64 ± 0. 12,7. 49 ±0. 07,and 4. 32 ± 0. 08,respectively; the relative mRNA expression levels of A3 A,A3 B,A3 C,A3 D,A3 F,A3 G,and A3 H in the adjacent normal tissues of HCC patients were 2. 93 ± 0. 11,4. 60 ± 0. 11,5. 48 ± 0. 11,5. 22 ± 0. 08,6. 97 ±0. 08,5. 91 ± 0. 12,and 4. 21 ± 0. 10,respectively; significant differences were found in the mRNA expression levels of A3 A,A3 B,A3 C and A3 G between the tumor tissues and adjacent normal tissues( all P 0. 05). The positive protein expression rates of A3 A,A3 B,A3 C,A3 D,A3 F,A3 G,and A3 H in the tumor tissues of HCC patients were 92. 3%,96. 8%,89. 0%,58. 1%,69. 7%,85. 9%,and 52. 9%,respectively; the positive protein expression rates of A3 A,A3 B,A3 C,A3 D,A3 F,A3 G,and A3 H in the adjacent normal tissues of HCC patients were 61. 3%,63. 2%,57. 4%,61. 9%,74. 8%,47. 7%,and 47. 7%,respectively; significant differences were found in the positive protein expression rates of A3 A,A3 B,A3 C and A3 G between the tumor tissues and adjacent normal tissues( all P 0. 05). The 5-year survival rate of patients with low expression of A3 A( 27. 3%) was higher than that in patients with high expression of A3 A( 17. 7%),with statistically significance( P 0. 05). The 5-year survival rate of patients with low expression of A3 B( 30. 0%) was higher than that in patients with high expression of A3 B( 17. 1%),with statistically significance( P 0. 05). The 5-year survival rate of patients with low expression of A3 G( 33. 6%) was higher than that in patients with high expression of A3 G( 13. 7%),with statistically significance( P 0. 05). Conclusions The mRNA and protein expression levels of APOBEC3 s-like cytidine deaminase gene family members such as A3 A,A3 B,A3 C,and A3 G are significantly upregulated in HCC tumor tissues. The survival rates of HCC patients with high expression of A3 A,A3 G,and A3 G are low.Detection of A3 A,A3 B,and A3 G mRNA levels in surgically resected tumor tissues of HCC patients can provide a reference for predicting and evaluating postoperative development and outcome.
【Fund】: 山东省自然科学基金资助项目(ZR2017PH012)
【CateGory Index】: R735.7
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