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《Shandong Medical Journal》 2018-47
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Expression of miR-218 and apoptosis-related proteins Bcl-2,Bax,xIAP and Survivin mRNA in gastric cancer

TAN Bibo;LI Yong;ZHAO Qun;FAN Liqiao;ZHAO Xuefeng;The Fourth Hospital of Hebei Medical University;  
Objective To observe the expression of micro RNA 218( miR-218) and apoptosis-related genes Bcl-2,Bax,x IAP,and Survivin mRNA in the gastric cancer tissues. Methods Eighty gastric cancer patients were enrolled in this study,and gastric cancer tissues,cancer-adjacent normal tissues were collected during operations,then real-time PCR was applied to test miR-128 and apoptosis-related genes Bcl-2,Bax,x IAP,and Survivin mRNA. Meanwhile,the correlation among these indexes was investigate. We used the mean value of the relative expression intensity of miR-218 in gastric cancer tissues as the boundary,and the gastric cancer patients were divided into the high miR-218 group and low miR-218 group,and then clinicopathological parameters and prognosis were also investigated. Results The levels of miR-218 and Bcl-2,Bax,x IAP,Survivin mRNA in the gastric cancer tissues were 0. 307 7 ± 0. 118 1,0. 817 7 ± 0. 272 6,0. 248 1 ±0. 110 8,0. 5995 ± 0. 2613,and 0. 820 9 ± 0. 342 6,respectively,and these indexes in the cancer-adjacent normal tissues were 0. 5659 ± 0. 226 0,0. 277 4 ± 0. 116 7,0. 444 4 ± 0. 201 9,0. 298 7 ± 0. 147 8,and 0. 247 0 ± 0. 112 2,respectively,with statistical difference( all P 0. 01). There was a negative correlation between miR-218 and Bcl-2 mRNA expression in the gastric cancer tissues( r =-0. 341,P 0. 01),and miR-218 was positively correlated with Bax mRNA expression( r = 0. 522,P 0. 01). Bcl-2 mRNA was negatively correlated with the expression of Bax mRNA( r =-0. 439,P 0. 01),and Bcl-2 mRNA was positively correlated with the expression of Survivin mRNA( r = 0. 235,P 0. 01). In 80 patients,42 cases were in the high miR-218 group,and 38 cases were in the low miR-218 group; there were 21 cases of stage Ⅰ + Ⅱ and 21 cases of stage Ⅲ + Ⅳ in the high miR-218 group,and there were 9 cases of stage I + II and 29 cases of stage Ⅲ + Ⅳ in the low miR-218 group,with significant difference( P 0. 05). Twenty-nine cases with lymphatic metastasis were verified in the high miR-218 group,and 34 cases with lymphatic metastasis were verified in the low miR-218 group,with significant difference( P 0. 05). The 5-year survival rates in the high miR-218 group and low miR-218 group were 66. 67% and 28. 95%,respectively,with significant difference( χ2= 9. 789,P 0. 01). Conclusion The miR-218 in gastric cancer is lowly expressed,miR-218 may be invovled in progression of gastric cancer by regulating Bcl-2,Bax,and survivin genes,and the low expression of miR-218 in gastric cancer tissues indicates that the tumor is in a high clinical stage,the possibility of lymph node metastasis is high,and the prognosis is poor.
【Fund】: 国家自然科学基金面上项目(81372580);; 河北省医学科学研究重点课题计划(20160176)
【CateGory Index】: R735.2
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