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《Acta Oceanologica Sinica》 2009-02
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Hemolytic activity of Karlodinium micrum at different growth phases and analysis of hemolysin species

ZHOU Cheng-xu1,FU Yong-jing1,CHEN Qing-fieng1,YAN Xiao-jun1(1.Ningbo University,Key Laboratory of Marine Biotechnology,Ningbo 315211,China)  
Hemolytic activity of the cell lysate and culture medium of Karlodinium micrum were tested at various algal growth phases,as well as to compare the difference between ethyl acetate and Bligh-Dyer,the major components of the hemolysin were fractionated with column chromatography and thin-layer chromatography.The results showed that the hemolytic activity in cells of K.micrum at the four growth phases maintained at a high level,whereas high hemolytic activity was only observed in the stationary and senescent phases in the filtrates the culture media,indicating that the hemolysin of K.micrum are normal physiological metabolites,and can be released into the environment when cells are in aging process.Ethyl acetate extraction and Bligh-Dyer extraction are both feasible to extract the hemolysin.Through chromatographic separation and hemolytic activity assay tracking,we found that there were three major hemolysin fractions in K.micrum,and two polyunsaturated fatty acid,namely 18∶5n3 and 22∶6n3,may play an important role in hemolysis.
【Fund】: 科技部中国欧盟科技合作项目(国科外〔2005〕262-0505);; 教育部长江学者与创新团队项目(IRT0734);; 宁波大学学科项目(XK0613046);; 国家科技支撑计划项目(2007BAD43B07)
【CateGory Index】: Q946
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