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《Acta Oceanologica Sinica》 2009-02
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Hemolytic activity of Karlodinium micrum at different growth phases and analysis of hemolysin species

ZHOU Cheng-xu1,FU Yong-jing1,CHEN Qing-fieng1,YAN Xiao-jun1(1.Ningbo University,Key Laboratory of Marine Biotechnology,Ningbo 315211,China)  
Hemolytic activity of the cell lysate and culture medium of Karlodinium micrum were tested at various algal growth phases,as well as to compare the difference between ethyl acetate and Bligh-Dyer,the major components of the hemolysin were fractionated with column chromatography and thin-layer chromatography.The results showed that the hemolytic activity in cells of K.micrum at the four growth phases maintained at a high level,whereas high hemolytic activity was only observed in the stationary and senescent phases in the filtrates the culture media,indicating that the hemolysin of K.micrum are normal physiological metabolites,and can be released into the environment when cells are in aging process.Ethyl acetate extraction and Bligh-Dyer extraction are both feasible to extract the hemolysin.Through chromatographic separation and hemolytic activity assay tracking,we found that there were three major hemolysin fractions in K.micrum,and two polyunsaturated fatty acid,namely 18∶5n3 and 22∶6n3,may play an important role in hemolysis.
【Fund】: 科技部中国欧盟科技合作项目(国科外〔2005〕262-0505);; 教育部长江学者与创新团队项目(IRT0734);; 宁波大学学科项目(XK0613046);; 国家科技支撑计划项目(2007BAD43B07)
【CateGory Index】: Q946
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【Citations】
Chinese Journal Full-text Database 5 Hits
1 ZHOU Chengxu, FU Yongjing, YAN Xiaojun ( Key Marine Biotechnology Lab, Ningbo University, Ningbo 315211, Zhejiang, China );Hemolytic Activity Inspection of a New Red Tide Organism Karlodinium micrum[J];Marine Science Bulletin;2007-03
2 ZHOU Cheng-xu,YAN Xiao-jun(Marine Biotechnology Key Laboratory,Ningbo University,Ningbo 315211,China);The Application of Tissue Culture Plate in Microalgae Collection[J];Ocean Technology;2006-04
3 PENG Xi-chun1, YANG Wei-dong2, LIU Jie-sheng2*,PENG Zhi-ying1, DENG Rui-xia2, JIANG Tian-jiu2 (1. Food and Biological Engineering College, South China University of Technology, Guangzhou 510640, China; 2. College of Life Sciences and Technology, Jinan University, Guangzhou 510632, China);Extraction of Haemolytic Substances from Alga Phaeocystis globosa[J];Journal of Tropical and Subtropical Botany;2005-01
4 PENG Xi-chuna, LIU Jie-shengb, YANG Wei-dongb(a. Department of Food Science and Technology, Jinan University; b. Department of Biological Science and Technology, Jinan University, Guangzhou 510632, China);Studies on Biosynthesis of Algal Toxins in Red Tide: A Review[J];Journal of Tropical and Subtropical Botany;2006-01
5 XU Ji-Lin, YAN Xiao-Jun, ZHOU Cheng-Xu, MA Bin and WANG Xiao-Shen (Key Laboratory of Marine Biotechnology, Ningbo University, Ningbo 315211);FATTY ACIDS STRESS RESPONSES OF A MARINE CYANOPHYTE PHORIDIUM SP. DURING DESALINATION PROCESS[J];Acta Hydrobiologica Sinica;2004-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 GUO Jin, YANG Weidong, LIU Jiesheng*,FAN Zhenhua Department of Biotechnology, Jinan University, Guangzhou 510632;Effects of salinity, temperature and light intensity on the growth and toxin production of Phaeocystis globosa[J];Acta Scientiae Circumstantiae;2007-08
2 ZHONG Na,YANG Weidong,LIU Jiesheng,ZHANG Jieling,HE Yang Department of Biotechnology,Jinan University,Guangzhou 510632;Effects of different nitrogen sources on the growth and toxin production of Prorocentrum lima[J];Acta Scientiae Circumstantiae;2008-06
3 YANG Weidong,LI Lixuan,LIU Jiesheng,ZHANG JianjunDepartment of Biotechnology,Jinan University,Guangzhou 510632;Allelopathy of marine benthic dinoflagellate-Prorocentrum lima on three red tide algae[J];Acta Scientiae Circumstantiae;2008-08
4 ZHOU Chengxu, SUN Xue, FENG Jing, YAN Xiaojun ( Marine Biotechnology Key Laboratory, Ningbo University, Ningbo, 315211, Zhejiang, China );Microscopic Observations and Molecular Identification of Toxic Unarmoured Dinoflagellates Karlodinium micrum (Dinophyceae) from the East China Sea (ECS)[J];Marine Science Bulletin;2008-03
5 GAO Li-juan, YANG Wei-dong, LIU Jie-sheng, Li Hong-Ye ( Department of Biotechnology, Jinan University, Guangzhou, 510632, China );Progress in the Study of Toxicological Mechanism of Chattonella marina[J];Marine Science Bulletin;2009-03
6 ZHANG Bin1, 2, YAN Tian1, CHEN Tao-Ying1, 2, ZHOU Ming-Jiang1 (1. Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071; 2. Graduate School, Chinese Academy of Sciences, Beijing, 100049);THE TOXICITY OF NON-PSP-PRODUCING ALEXANDRIUM AFFINE[J];Oceanologia Et Limnologia Sinica;2008-04
7 ;Progress on study of competition among marine microalgae[J];Marine Sciences;2007-05
8 TAN Zhi-jun1,2,YAN Tian 1,YU Ren-cheng 1,ZHOU Ming-jiang 1(1.Institute of Oceanology,Chinese Academy of Science,Qingdao 266071,China;2.Graduate School,Chinese Academy of Science,Beijing 100039,China);The preliminary research on the hemolytic activity of the dinoflagellate Alexandrium spp.[J];Marine Sciences;2008-12
9 LIU Jie-sheng,XIE Jin,YANG Wei-dong (Department of Biotechnology,Guangzhou 510632,China);Studies on haemolytic toxins action in allelopathy of Alexandrium tamarense[J];Journal of Jinan University(Natural Science Edition);2007-01
10 XU Ji-lin1,2,YAN Xiao-jun2,ZHOU Cheng-xu2,MA Bin2,TANG Xue-xi1(1.College of Marine Life Science,Ocean University of China,Qingdao 266003,China;2.Key Laboratory of Marine Biotechnology,Ningbo University,Ningbo 315211,China);The Effects of Air Bubbling Condition on Fatty Acid Composition in 19 Strains of Marine Microalgae[J];Journal of Ningbo University(Natural Science & Engineering Edition);2006-02
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 CHEN Yong, LIU Jie-sheng, LUO Ying, et al. College of Life Science and Technol-ogy, Ji'nan University, Guangzhou 510632, China;Preliminary Research on the Methods of Fragmentation of the Cell Wall and the Toxicity of Alexandrium tamarense in Different Growth Phases[J];Journal of Environment and Health;2002-03
2 QI Yu-zao, SHEN Ping-ping, WANG Yan (Institute of Hydrobiology, Jinan University, Guangzhou 510632, China);TAXONOMY AND LIFECYCLE OF GENUS PHAEOCYSTIS (PRYMNESIOPHYCEAE)[J];Journal of Tropical and Subtropical Botany;2001-02
3 LU Kai-hong and LIN Xia (Oceanographic & fisheries department, Ningbo University, 315211);THE EFFECT OF DOMESTICATION WITH SEAWATER AND FRESHWATER ON FATTY ACID COMPOSITION OF FIVE SPECIES OF MICROALGAE[J];Acta Hydrobiologica Sinica;2001-02
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