Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Practical Obstetrics and Gynecology》 2014-03
Add to Favorite Get Latest Update

Effect of Quercetin and Heparin Enzyme on Invasion and Metastasis of Cervical Cancer Caski Cells

CHENG Jing;ZHANG Wei;LV Qiongying;Renmin Hospital of Wuhan University;  
Objective:To evaluate the effect of heparan enzyme(heparanase HPA) and quercetin on invasion and metastasis of cervical cancer Caski cells through the transwell chamber and substrate scratch damage methods.Methods:According to the HPA gene nucleotide sequence in GenBank database,we design 4 shRNA oligonucleotide sequences targeting HPA,and cloned into the pYr-1.1 plasmid vector.One shRNA sequence was selected and transfected into cervical cancer Caski cells.Four groups were assigned as follows:control group,quercetin treatment group,HPA-ShRNA(group and HPA-ShRNA+quercetin group.The invasion and metastasis abilityof cervical cancer cells were observed through Trans well chamber substrate scratch damage experiment.Results:After treatment duration of 6,12,24 hours,the migration ability in quercetin treatment group and HPA-shRNA group decreased significantly in comparison with control group(P0.05 and P0.01),but no difference between quercetin treatment group and HPA-shRNA group(P0.05).The migration ability in HPA-shRNA+quercetin group decreased significantly in comparison with the other 3 groups(P0.01).The invasion ability in quercetin treatment group and HPA-shRNA group decreased significantly in comparison with control group(P0.05).The invasion ability in HPA-shRNA+quercetin group decreased significantly in comparison with the other 3 groups(P0.01) Conclusions:Quercetin could obviously inhibit the ability of invasion and metastasis of cervical cancer Caski cells.HPA was associated with the process of invasion and metastasis of cervical cancer cells,which might be involved in effect of quercetin.
【Fund】: 国家自然科学基金项目(编号:31170329)
【CateGory Index】: R737.33
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 LU Chuang-xin*,ZHOU Yun,XUE Fei,WANG Wen-yu,LI Xiao-yan,GUO Zhi-song(*Department of Oncology,People's Hospital of Zhengzhou University,Zhengzhou 450003,China);Antiproliferative and apoptogenic activities of quercetin on HepG2 cells[J];Journal of Chinese Practical Diagnosis and Therapy;2012-08
2 MA Shuang-wei,WU Kai-nan,ZHONG Xiao-gang,KONG Ling-quan Department of General Surgery,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,P.R.China;Study of the inhibitory effect of quercetin on transplantation tumor of breast carcinoma cell line MCF-7 in nude mice[J];Journal of Qilu Oncology;2004-03
3 Zhang Jie Lü Cai Zhang Ping-an et al Department of Urology, People's Hospital of Wuhan University, Wuhan;Empirical Studies on Quercetin for Inhibition of Proliferation ofhuman Uterine Cervix Cancer HeLa cells[J];Chinese Journal of Clinical Oncology;2006-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 GU Sheng-jiu et al(Guilin Medical University,Guilin,Guangxi 541004);Intervention Effects of Quercetin from Sophora japonica on the Human Nasopharyngeal Carcinoma Cell[J];Journal of Anhui Agricultural Sciences;2012-18
2 Wang Cuiping,Ma Li,Xie Jingjing Department of Obstetrics and Gynecology,the first Hospital of Anqing,Anqing 246003;The analysis of colposcopy usage in diagnoses of 734 cases cervical diseases[J];Anhui Medical Journal;2009-08
3 Zhang Xun 1 ,Li Ling 1 ,Zhang Wen-Hua 2 ,Li Shu-Min 2;Evaluation of Cold Knife Conization(CKC)on Diagnosis and Treatment of Cervical Intraepit helial Neoplasia[J];Chinese Journal of Cancer;2003-09
4 ZHOU Ping (Department of Obstetrics and Gynecology,The First People's Hospital of Huainan,Huainan Anhui 232007,China);Use of high-frequency electric-wave knives in treatment of cervical pathological changes[J];Journal of Bengbu Medical College;2008-04
5 LI Qun,TANG Zhao-qian,GUO Su-yang(Department of Gynecologic Oncology,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,China);Treatment of cervical intraepithelial neoplasia and cervical cancer Ⅰa_1 by cervical conization:analysis of 99 cases[J];Journal of Bengbu Medical College;2009-01
6 LI Jie(Department of Obstetrics and Gynecology,Anhui Provincally Friendship Hospital,Hefei Anhui 230011,China);The significance of ThinPrep cytologic test combined colposcopy with biopsy in cervical carcinoma investigation[J];Journal of Bengbu Medical College;2009-07
7 ZHU Yun-ju,WEI Ming-xiu(Department of Obstetrics,Luwan Branch,Ruijin Hospital,Affiliated to Shanghai Jiao Tong Universtiy School of Medicine,Shanghai 200020,China);Colposcopy in diagnosis of cervical disease[J];Journal of Bengbu Medical College;2010-02
8 LI Xu,DU Dan-li,JIN Mao-sheng(Department of Obstetrics and Gynecology,The First Affiliated Hospital of Bengbu Medical College,Bengbu Anhui 233004,China);Loop electrosurgical excision procedure for treatment of cervical lesions:a report of 107 cases[J];Journal of Bengbu Medical College;2010-12
9 JIANG CHANG-QING,SONG MEI,WANG XIA, et al (Department of Pathology,Qingdao Municipal Hospital,Qingdao 266011,China);EVALUATION OF LOOP ELECTROSURGICAL EXCISION IN THE DIAGNOSIS AND TREATMENT OF CERVICAL LESIONS[J];Acta Academiae Medicinae Qingdao Universitatis;2007-02
10 WANG LEI,WANG FU-LING,LIU LI-ZHI,et al(Department of Obestetrics and Gynecology,The Affiliated Hospital of Qingdao University Medical College,Qingdao 266003,China);THE APPLICATION OF LIQUID-BASED CYTOLOGICAL THIN SMEAR TECHNIQUE IN SCREENING CERVIX LESIONS[J];Acta Academiae Medicinae Qingdao Universitatis;2007-03
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 WANG Zhenzhen,QUAN Peiliang,XU Hong,et al.Henan Tumor Hospital,Zhengzhou 450003,China;Expression of survivin in non-small cell lung cancer and its correlation with bcl-2[J];Journal of Chinese Practical Diagnosis and Therapy;2010-07
2 LI Shi-jun1),SHI Guang-xia2),YUAN Hong1),WANG Xiao-yan1)1) Department of Clinical Laboratory,the First Affiliated Hospital of Dalian Medical University,Dalian 116011,China;2) Staff Room of Pathophysiology of Dalian Medical University,Dalian 116044,China;Expression of apoptosis stimulating protein of P53 in non-small cell lung cancer[J];Journal of Chinese Practical Diagnosis and Therapy;2010-11
3 ZHANG Yan-bo1),WANG Jun1),WANG Yong2),LI Shuang3),WANG Ling-yu4),ZHOU Guo-qing1) 1) Department of Neurology, Nanxishan Hospital,Guilin 541001,China;2) Department of Pathophysiology,School of Basic Medical of Guilin Medical College,Guilin 541002,China;3) Department of Laboratory,Nanxishan Hospital,Guilin 541002,China;4) Department of Life Sciences,School of Basic Medical of Guilin Medical College,Guilin 541002,China;Effect of berberine on expression and apoptosis of Bcl-2 and Bax in cerebral ischemia reperfusion in rats[J];Journal of Chinese Practical Diagnosis and Therapy;2011-02
4 CHEN Xue-mei1,CHEN Ji-ming2,LI Si-yaun2(1.Department of Gynecology and Obstetrics,Yanqi Hospital of The Second Agricultural Division of Xinjiang,Xinjiang Bazhou 841000,China;2.Department of Gynecology and Obstetrics,The First Affiliated Hospital of Medical College,Shihezi University,Xinjiang Shihezi 832000,China);Progress in research on screening methods of cervical cancer[J];Chinese Journal of Woman and Child Health Research;2007-02
5 Kong Lingquan Wu Kainan Lin Hui Department of Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing;Study of Inhibitory Effect of Quercetin on Neovascilarization of Experimental Mammary Carcinoma[J];Chinese Journal of Clinical Oncology;2001-04
6 CHEN Jiao,ZHANG Wei,AO Liang-fei,WANG Dan-li,YU Zhen-gui.Hospital of Wuhan University/Community Health Center of Wuhan University,Wuhan 430072,China;Effect of quercetin on the expression of STAT3 and its signal pathway in HeLa cells[J];Chinese Journal of Clinicians(Electronic Edition);2011-19
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved