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《Journal of Practical Obstetrics and Gynecology》 2014-03
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Effect of Quercetin and Heparin Enzyme on Invasion and Metastasis of Cervical Cancer Caski Cells

CHENG Jing;ZHANG Wei;LV Qiongying;Renmin Hospital of Wuhan University;  
Objective:To evaluate the effect of heparan enzyme(heparanase HPA) and quercetin on invasion and metastasis of cervical cancer Caski cells through the transwell chamber and substrate scratch damage methods.Methods:According to the HPA gene nucleotide sequence in GenBank database,we design 4 shRNA oligonucleotide sequences targeting HPA,and cloned into the pYr-1.1 plasmid vector.One shRNA sequence was selected and transfected into cervical cancer Caski cells.Four groups were assigned as follows:control group,quercetin treatment group,HPA-ShRNA(group and HPA-ShRNA+quercetin group.The invasion and metastasis abilityof cervical cancer cells were observed through Trans well chamber substrate scratch damage experiment.Results:After treatment duration of 6,12,24 hours,the migration ability in quercetin treatment group and HPA-shRNA group decreased significantly in comparison with control group(P0.05 and P0.01),but no difference between quercetin treatment group and HPA-shRNA group(P0.05).The migration ability in HPA-shRNA+quercetin group decreased significantly in comparison with the other 3 groups(P0.01).The invasion ability in quercetin treatment group and HPA-shRNA group decreased significantly in comparison with control group(P0.05).The invasion ability in HPA-shRNA+quercetin group decreased significantly in comparison with the other 3 groups(P0.01) Conclusions:Quercetin could obviously inhibit the ability of invasion and metastasis of cervical cancer Caski cells.HPA was associated with the process of invasion and metastasis of cervical cancer cells,which might be involved in effect of quercetin.
【Fund】: 国家自然科学基金项目(编号:31170329)
【CateGory Index】: R737.33
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