Anti-tumor Activity of Glycosaminoglycan from Ruditapes philippinarum
WANG Ya-nan, FAN Xiu-ping, WU Hong-mian (College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524025,China)
CRG was extracted from Ruditapes philippinarum using two-enzymatic hydrolysis and was purified by DEAE-52 cellulose chromatography. MTT colorimetric assay was carried out to evaluate CRG and RG's anti-tumor effect on human hepatoma cell line Bel7402 in vitro. The anti-tumor activity of CRG in vivo was evaluated by calculating the inhibitory rates of S180 tumor, meanwhile, the thymus and spleen indexes were measured. The thymus and spleen indexes and the carbon clearance abilities of normal and immunosuppressional mice were also measured. The results showed that CRG and RG could inhibite the growth of human hepatoma cell line Bel7402 in vivo and the effect of RG was better than that of CRG.. CRG could significantly inhibit the growth of S180 tumor and splenomegaly caused by tumor. CRG could also antagonize the thymus atrophy and spleen atrophy induced by cyclophosphamide.
【CateGory Index】： R730.5