Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Guangdong Ocean University》 2009-03
Add to Favorite Get Latest Update

Effect of Several Factors on Motility of Sperm of Epinephelus lanceolatus

LIANG Wei-feng,ZHANG Hai-fa,WANG Yun-xing,HUANG Guo-guang,HUANG Pei-wei,SHEN Nan-nan (Guangdong Baya Bay Fisheries Development Center,Huizhou 516081,China)  
Salinity,pH,temperature and preservation time were used to assess the sperm viability of Epinephelus lanceolatus by means of different scales of those factors,and as the experiments on process,the optimal value of the front factors set as the following experiment conditions. The optimal motility was obtained at salinity between 27.5-35.0,with the longest viability at 32.5 recording the motility time and duration of 13.2 and 14.8 min,respectively. With respect to pH,the longest time of fast motility and duration was acquired,when the value was 8.5,with the motility time and duration of 12.7 and 17.3 min,respectively. A maximum motility time of 16.6min was recorded in 29 ℃,with the best suitable temperature ranging 27 -31℃. Of all the three factors,the sperm optimal viability requirement was the same as that of the breeding conditions. After preserved at temperature 0 -4 ℃ for 6 hours the motility time of sperm was 18.2 min,which was not significantly reduced compared with the initial time 18.4min. After 120 hours stored at the same temperature,the motility time of sperm was 2.3 min and the activation rate was 3.3%. The viability debased following the preservation time calculated,suggesting the sperm should be used as soon as possible after preserved. The motility time ( y ) and duration ( y' ) fitted the equations:y = -0.8334 x + 19.164,R2 = 0.9856; y' = -1.1358 x + 27.8,R2 = 0.9573.
【Key Words】: Epinephelus lanceolatus sperm viability salinity pH temperature preservation time
【Fund】: 国家高技术研究发展计划(863计划)(2006AA10A414-2);; 广东省重大科技兴海项目(A200600D03);; 广东省科技计划项目(2006B20201062)
【CateGory Index】: S917.4
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 6 Hits
1 SHU Hu~1, HUANG Yan~1, ZHANG Hai-fa~2, WANG Yun-xin~2, LIUFU Yong-zhong~2(1. School of Biology and Chemistry Engineering, Guangzhou University, Guangzhou 510405,China ;2.Guangdong Dayawan Fishery Development Center, Huizhou 516081, China);The effect of salinity and temperatures on sperm motility of Lutjanus erythopterus[J];Journal of Guangzhou University(Natural Science Edition);2005-01
2 DENG Yue song, LIN Hao ran (School of Life Science, Zhongshan University, Guangzhou 510275, Guangdong ,China);Advance in Fishes Sperm Motility[J];LIFE SCIENCE RESEARCH;1999-04
3 XIE Gang; YE Xing;SU Zhi-Peng; YU De-Guang; PAN De-Bo (Pearl River Fishery Research Institute, CAFS, Guangzhou 510380);THE MAIN BIOLOGICAL CHARACTERISTICS OF SPERM OF ANGUILLA JAPONICA[J];JOURNAL OF SHANGHAI FISHERIES UNIVERSITY;1999-01
4 JIANG Shi\|Gui,LI Jia Er,OU You\|Jun,ZHENG Yun\|Tong (South China Sea Fisheries Research Institute,CAFS,Guangzhou 510300);Relationships between conditions for activating spermatozoa of four sparidae fishes and the fishes'ecological habits[J];ACTA ECOLOGICA SINICA;2000-03
5 Pan Debo Xu Shuying Ye Xing Xie Gang Pang Shixun Qi Baolun Su Zhifeng (Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences,Guangzhou 510380);Study on the main biological characteristics of sperm of Megalobrama hoffmanni[J];JOURNAL OF FISHERY SCIENCES OF CHINA;1999-04
6 ZHAO Huihong1,2, LIU Xiaochun1, LIN Haoran1, LIUFU Yongzhong1,3, Wang Yunxin3f Guangdong Province for Aquatic Economic Animals,Zhongshan University, Guangzhou 510275, China; 2. Department of Fisheries, School of Animal Science, South ChinaAgricultural University, Guangzhou 510642, China; 3. Guangdong Daya Bay Fishery Development Center, Huizhou 516081, China);Ultrastructure of spermatozoa and effects of salinity, temperature and pH on spermatozoa motility in Epiniphelus coioides[J];Journal of Fishery Sciences of China;2003-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Wen-hai(School of Life Science,Hunan University of Science and Technology,Xiangtan,Hunan 411201);Research Progress on Artificial Gynogenesis in Fish[J];Journal of Anhui Agricultural Sciences;2008-35
2 WAN Quan et al(College of Animal Science and Technology,Anhui Agricultural University,Hefei,Anhui 230036);Study on the Application of Spermatozoa Preservation at Low Temperature in Artificial Fecundation of Myxocyprinus asiaticus Spermatozoa[J];Journal of Anhui Agricultural Sciences;2009-03
3 TIAN Zhao-hui et al(National Engineering Research Center for Freshwaters and Beijing Fisheries Research Institute,Beijing 100068);Study on Gonadal Development of Brycon cephalus Cultured in Cement Pool[J];Journal of Anhui Agricultural Sciences;2009-18
4 LI Fei1,WAN Quan1,ZHUANG Yong-long2,SHEN Bao-ping3(1.School of Animal Science and Technology,Anhui Agricultural University,Hefei 230036;2.Biotechnology Center,Anhui University,Hefei 230039;3.Anhui Provincial Changjiang River Aquatic Animal Protection and Research Center,Wuwei 238300);Structure of Myxocyprinus asiaticus(Bleeker)spermatozoon[J];Journal of Anhui Agricultural University;2009-02
5 YOU Yong-Long **,LIN Dan-Jun,SU Min Department of Biology, Fujian Normal University, Fuzhou 350007, China;The formation of nuage in the oogenesis of the teleost Spinibarbus caldwelli (Nichols)[J];Acta Zoologica Sinica;2004-02
6 WU Jin-Ying,LIN Hao-Ran Key Laboratory of Guangdong for Improved Variety Reproduction of Aquatic Economic Animal,Institute of Aquatic Economic Animals,School of Life Sciences,Zhongshan University,Guangzhou 510275,China;Microstructure and ultrastructure of the thymus of orange-spotted groupers Epinephelus coioides[J];Acta Zoologica Sinica;2008-02
7 WU Jian Feng ①② HUA Yuan Yu ② Zou Hong Hai ② (① Jiangsu Province Nangtong Normal School,Nantong 226006; ②College of Biological Science, Nanjing Normal University,Nanjing 210097,China);Artificial Breeding Techniques for Captive Takifugu obscurus[J];Chinese Journal of Zoology;2003-01
8 CAO Yun-Chang①② LI Wen-Sheng① YE Wei③ LIN Hao-Ran①(①Institute of Aquatic Economical Animal & Guangdong Provincial Key Laboratory for Aquatic Economical Animal,Zhongshan University,Guangzhou 510275;②School of Life Sciences & Biotechnology,University of South China,Hengyang 421001;③National Tilapia Breeding Farm of Guangdong Province,Guangzhou 511453,China);Gonadal Development in Green Sunfish,Lepomis cyanellus:A Histological Study[J];Chinese Journal of Zoology;2008-01
9 LIANG Gang YE Xiao-Lin REN Yao-Hui(College of Life Science,Shaanxi Normal University,Xi′an 710062, China);Microstructural Changes on Granulosa Cells during Follicular Development in Zaocys dhumnades[J];Chinese Journal of Zoology;2009-01
10 TIAN Zhao-hui1,HU Hong-xia1,BAI Yan2,ZHU Hua1(1.National Engineering Research Center for Freshwater Fisheries(Beijing) and Beijing Fisheries Research Institute,Beijing 100068,China;2.Animal Husbandry and Veterinary Medicine College,Liaoning Medicinal University,Jinzhou 121001,China);Histological Observation of Gonadal Development in Ayu(Plecoglossus altivelis) Cultured in Ponds[J];Fisheries Science;2007-11
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LI Jiaer; OU Youjnn; JIANG Shigui(South China Sea Fisheries Institute, CAFS Guangzhou 510300);EFFECT OF ENVIRONMENT CHANGE OF THE VITALITY OF SPERMATOZOA IN RHABDORSARGUS SARBA[J];CHINESE JOURNAL OF ZOOLOGY;1996-03
2 Hong Wanshu, Zhang Qiyong and Zhou Dongchen (Oceanography Department, Xiamen University, 361005);RELATIONSHIP BETWEEN ENVIRONMENTAL FACTORS AND PHYSIOLOGICAL CHARACTERISTICS OF FOUR COMMERCIAL SEAWATER FISH SPERMATOZOA[J];MARINE SCIENCES;1997-03
3 Jiang Shigui 1, Li Jiaer 1, Ou Youjun 1, Zhong Chuangguang 2 and Yu Dahui 1 ( 1South China Sea Fish, Institute, CAFS, Guangzhou, 510300) ( 2Biology Department, Zhongshan University, Guangzhou, 510275);STUDIES ON HYBRIDIZATION BETWEEN FEMALE SILVER BREAM Rhabdosargus sarba AND MALE RED BREAM Pagrosomus major[J];MARINE SCIENCES;1997-05
4 OU Youjun; Li Jiaer; Jiang Shigui (South China Sea Fisheries Institute, The Chinese Academy of Fishery Sciences, Guangzhou 510300);EFFECTS OF PRESERVATION AND ACTIVATION ON BIOLOGICAL PROPERTY OF PAGROSOMUS MAJOR (TEMMINCK ET SCHLEGEL) SPERMATOZOA[J];TROPIC OCEANOLOGY;1998-03
5 Jiang Shigui; Li Jiaer; On Youjun; Zheng Yuntong (South China Sea Fisheries Institute, CAFS, Guangzhou, 510300);Comparative study on the Adaptive Salinity Ranges for Activating Spermatozoa of Four Sparidae Fishes from South China Sea[J];JOURNAL OF ZHANJIANG OCEAN UNIVERSITY;1998-04
6 PANG Shi-Xun; XIE Gang; XU Shu-Ying; YE Xing;SU Zhi-Feng; PAN De-Bo; QI Bao-Lun (Pearl River Fishery Research Institute, CAFS, Guangzhou 510380)LIN Lang(Shunde City Zhuotan Cultured Farm of Guangdong Province, 528318);STUDIES ON THE WHOLE ARTIFICIAL PROPAGATION OF MEGALOBRAMA HOFFMANNI[J];JOURNAL OF SHANGHAI FISHERIES UNIVERSITY;1998-01
7 Zhang Yaoguang, Luo Quansheng and Zhong Mingchao (Department of Biology, Southwest China Teachers University, Chongqing 630715);STUDIES ON THE STRUCTURE OF TESTIS AND SPERMATOZOON IN LEIOCASSIS LONGIROSTRIS[J];Acta Hydrobiologica Sinica;1993-03
8 JIANG Shi\|Gui,LI Jia Er,OU You\|Jun,ZHENG Yun\|Tong (South China Sea Fisheries Research Institute,CAFS,Guangzhou 510300);Relationships between conditions for activating spermatozoa of four sparidae fishes and the fishes'ecological habits[J];ACTA ECOLOGICA SINICA;2000-03
9 Pan Debo Xu Shuying Ye Xing Xie Gang Pang Shixun Qi Baolun Su Zhifeng (Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences,Guangzhou 510380);Study on the main biological characteristics of sperm of Megalobrama hoffmanni[J];JOURNAL OF FISHERY SCIENCES OF CHINA;1999-04
10 ZHAO Huihong1,2, LIU Xiaochun1, LIN Haoran1, LIUFU Yongzhong1,3, Wang Yunxin3f Guangdong Province for Aquatic Economic Animals,Zhongshan University, Guangzhou 510275, China; 2. Department of Fisheries, School of Animal Science, South ChinaAgricultural University, Guangzhou 510642, China; 3. Guangdong Daya Bay Fishery Development Center, Huizhou 516081, China);Ultrastructure of spermatozoa and effects of salinity, temperature and pH on spermatozoa motility in Epiniphelus coioides[J];Journal of Fishery Sciences of China;2003-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved