Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Universitatis Medicinalis Secondae Shanghai》 2004-01
Add to Favorite Get Latest Update

Cytotoxicity effect of matrine on gastric cancer cell lines in vitro

ZENG Hui , HU Mei-jie , ZHANG Shu , QIAO Mim-min , ZHANG Yong-ping, SHI Gui-ying, WU Yun-lin (Department of Gastroenterology, Ruijin Hospital, Shanghai Second Medical University, Shanghai 200025 , China; Deparment of Cell Biology, College of Basic Medicine,Shanghai Second Medical University)  
Objective To study the cytotoxicity effect of matrine and its mechanism on gastric cancer cell lines in different differentiations. Methods Matrine in different doses were used to treat moderate (SGC-7901) and lower (AGS) differentiated gastric cancer cell lines for 24、48 and 72 h respectively , and MTT assay was employed to evaluate the cytotoxicity of matrine on these tumor cell lines; meanwhile, transmission electron microscopy and flow cytometry were utilized to detect the apoptosis of SGC-7901 induced by matrine. Results Following the increase in the concentration of the drug and prolongation of administration times, the cytotoxicity of matrine on both kinds of gastric cancer cell lines were enhanced significantly(P0. 01) ; especially the lower differentiated AGS was more sensitive to matrine than the moderate SGC-7901. Using transmission electron microscopy and flow cytometry, apoptosis induced by matrine was confirmed in SGC-7901. Conclusion The cytotoxicity of matrine on gastric cancer cell lines, in connection with its apoptosis-inducing effects, is dose and time dependent significantly; the lower differentiated gastric cancer cell line is more sensitive to matrine than the moderate one.
【Fund】: 上海市自然科学基金资助项目(01ZB14025)
【CateGory Index】: R735.2
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved