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《Journal of Shanghai Jiaotong University》 2008-05
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The Effects of Mercaptoacetic Acid Modified CdTe Quantum Dots on Specificity of Polymerase Chain Reaction

LI Qing,HE Rong,GAO Feng,PAN Bi-feng,CUI Da-xiang(Institute of Micro and Nano Science and Technology,National Key Laboratory of Nano/Micro Fabrication Technology,Key Laboratory for Thin Film and Microfabrication of Ministry of Education,Shanghai Jiaotong University,Shanghai 200240,China)  
The effects of mercaptoacetic acid(TGA) modified CdTe quantum dots on the specificity of polymerase chain reaction(PCR) were explored.TGA modified CdTe quantum dots were synthesized.XRD(analysis) shows that cubic CdTe QDs are formed.TGA modified CdTe QDs were synthesized with the diameter of 3nm from HR-TEM observation and surface negative charge.TGA modified CdTe quantum dots were added into PCR liquids,brcaa1 gene vector was used as PCR template,two-round PCRs were done.The final PCR products were analyzed by 1% agarose gel electrophoresis.1% agarose gel electrophoresis analysis shows that below the concentration of 1.33 mg/ml CdTe QDs,as the amounts of QDs increase in the PCR liquids,the non-specific amplification bands gradually disappear.PL shows that photoluminesence(PL) intensity of PCR liquids after PCR is lower than that before PCR.XPS shows that no element position shift is observed.UV-vis spectroscopy analysis shows that UV-vis absorption spectra after PCR is higher than that before PCR.TGA modified CdTe quantum dots in PCR liquids can markedly improve PCR specificity.The phenomena owns potential in application such as ultra-sensitive DNA detection,photoelectric biosensors.
【Fund】: 国家重点基础研究发展规划(973)项目(2005CB723400-G);; 国家自然科学基金(20771075);; 上海市浦江人才计划(06PJ14049);; 上海市纳米专项(0752nm024)资助项目
【CateGory Index】: Q50
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