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Effects of bFGF on Biological Characteristics of Cells Derived from Periodontium

GE Shao hua,YANG Pi shan. (Hospital of Stomatology attached to Shangdong Medical University,Shandong 250012,China)[  
Objective To observe effects of basic fibroblast growth factor on the biological characteristics of cultured human gingival,periodontal ligament fibroblasts and human alveolar bone cells, such as proliferation, alkaline phosphatase activity, protein synthesis and the formation of mineralized nodules. Methods Using cell culture technique, MTT colorimetric assay, ALP activity assay, Commasie brilliant blue staining and Dahl McGec Russell's alizarin red stain for calcium.Results bFGF enhanced the proliforative responses of the three types of cells. In contrast, bFGF inhabited the induction of alkaline phosphatase activity, protein synthesis and the mineralized nodule formation by PDLF and ABC. Conclusion bFGF can enhance cell proliferation while inhibit cytodifferentiation, thus accelerating periodontal regeneration. [
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