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《Shanghai Journal of Stomatology》 2004-05
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Effect of static magnetic field on proliferation and cell cycle of osteoblast cell

QIU Li-hong, TANG Xu-na, ZHONG Ming, WANG Zhao-yuan. (College of Stomatology, China Medical University. Shenyang 110002, Liaoning Province, China)  
Rat calvarias osteoblasts were cultured and put in the static magnetic field of 0Gs, 400Gs, 620Gs, 830Gs and 1080Gs for 24h, 48h and 72h, respectively. MTT method was used for detecting osteoblast proliferation and flow cytometry for detecting cell cycle. When the magnetic exposure time extended to 48h or 72h, the corresponding OD value of osteoblast with magnetic treatment of 400 Gs or 620 Gs increased obviously with significant difference as compared with 0Gs group (P 0.05), but such proliferative effect was not shown in the 1080 Gs group (P 0.05). After magnetic exposure with intensity of 620 Gs, G0/G1 phase percentage decreased, but S phase and G2/M phase percentage was significantly higher as compared with control. Proliferation index (PI) increased as well and there was significant difference in contrast to control (P 0.05). Within a certain range of magnetic intensity, magnetic field enhances cell growth in a dose-dependent pattern. And static magnetic field can activate static G1 phase into S phase, increase DNA synthesis, and accelerate cell proliferation.
【Fund】: 辽宁省教育厅基金(20122170)
【CateGory Index】: R35
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