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Effect of static magnetic field on bone morphogenetic protein 2 and collagen type I of osteoblast cell

QIU Li-hong,ZHONG Ming,TANG Xu-na,WANG Zhao-yuan.(College of Stomatology,Chinese Medical University.Shenyang 110002,Liaoning Province,China)  
PURPOSE:To study the effect of static magnetic field on osteoblast,and to explore the possibility of osteogenesis of static magnetic field.METHODS:Rat calvarial osteoblasts were seeded on the 96-well culture plate,and exposed to 0.062T magnetic intensity field for 24 hours,48 hours and 72 hours,respectively.Changes of BMP-2 and collagen type I in osteoblast were examined by Western blot.Immunohistochemical staining was carried out at different time periods.The data obtained were analyzed for ANOVA using SPSS 11.5 software package.RESULTS:The results of Western blot showed that magnetic static field affected expression of BMP-2 and collagen type I in osteoblast.The expression of BMP-2 in 48-hour group increased 2.1 fold and the expression of collagen type I in 72-hour group increased 1.6 fold compared to the control group(P0.01).Moreover,the staining color of the collagen type I in cell of magnetic treated group was deeper than that of the control.CONCLUSIONS:Static magnetic field induced the expression of BMP-2 and stimulated secretion of collagen type I.The results indicate that osteoblasts are sensitive to 0.062T static magnetic field stimulation.
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