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《Shanghai Journal of Stomatology》 2009-02
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Effects of lipopolysaccharides extracted from Porphyromonas endodontalis on the expression of IL-1β mRNA and IL-6 mRNA in osteoblasts

YANG Di,LI Ren,QIU Li-hong,LI Chen.(Department of Endodontics, College of Stomatology,China Medical University.Shenyang 110002,Liaoning Province,China)  
PURPOSE: To quantify the IL-1β mRNA and IL-6 mRNA expression induced by lipopolysaccharides (LPS) extracted from Porphyromonas endodontalis(P.e) in osteoblasts, and to relate P.e-LPS to bone absorption pathogenesis in lesions of chronical apical periodontitis. METHODS: MG63 was treated with different concentrations of P.e-LPS(0-50μg/ mL) for different hours (0-24h). The expression of IL-1β mRNA and IL-6 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR).Statistical analysis was performed using one- way ANOVA and Dunnett t test with SPSS11.0 software package. RESULTS: The level of IL-1β mRNA and IL-6 mRNA increased significantly after treatment with P.e-LPS at more than 5μg/mL(P0.01)and for more than 1 hour(P0.01) ,which indicated that P.e-LPS induced osteoblasts to express IL-1β mRNA and IL-6 mRNA in dose and time dependent manners. CONCLUSION: P. e -LPS may promote bone resorption in lesions of chronical apical periodontitis by inducing IL-1β mRNA and IL-6 mRNA expression in osteoblasts.
【Fund】: 辽宁省高等学校科研计划项目(2008819)~~
【CateGory Index】: R78
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