Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Shanghai Journal of Stomatology》 2009-02
Add to Favorite Get Latest Update

Effects of lipopolysaccharides extracted from Porphyromonas endodontalis on the expression of IL-1β mRNA and IL-6 mRNA in osteoblasts

YANG Di,LI Ren,QIU Li-hong,LI Chen.(Department of Endodontics, College of Stomatology,China Medical University.Shenyang 110002,Liaoning Province,China)  
PURPOSE: To quantify the IL-1β mRNA and IL-6 mRNA expression induced by lipopolysaccharides (LPS) extracted from Porphyromonas endodontalis(P.e) in osteoblasts, and to relate P.e-LPS to bone absorption pathogenesis in lesions of chronical apical periodontitis. METHODS: MG63 was treated with different concentrations of P.e-LPS(0-50μg/ mL) for different hours (0-24h). The expression of IL-1β mRNA and IL-6 mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR).Statistical analysis was performed using one- way ANOVA and Dunnett t test with SPSS11.0 software package. RESULTS: The level of IL-1β mRNA and IL-6 mRNA increased significantly after treatment with P.e-LPS at more than 5μg/mL(P0.01)and for more than 1 hour(P0.01) ,which indicated that P.e-LPS induced osteoblasts to express IL-1β mRNA and IL-6 mRNA in dose and time dependent manners. CONCLUSION: P. e -LPS may promote bone resorption in lesions of chronical apical periodontitis by inducing IL-1β mRNA and IL-6 mRNA expression in osteoblasts.
【Fund】: 辽宁省高等学校科研计划项目(2008819)~~
【CateGory Index】: R78
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 3 Hits
1 QIU Li-hong,ZHONG Ming,TANG Xu-na,WANG Zhao-yuan.(College of Stomatology,Chinese Medical University.Shenyang 110002,Liaoning Province,China);Effect of static magnetic field on bone morphogenetic protein 2 and collagen type I of osteoblast cell[J];Shanghai Journal of Stomatology;2007-01
2 SUN Ying,SHU Rong,ZHANG Ming-zhu,LI Chao-lun.(Department of Periodontology,Shanghai Key Laboratory of Stomatology,Ninth People's Hospital,College of Stomatology,School of Medicine,Shanghai Jiao Tong University.Shanghai 200011,China);Effect of TLR4 on expressions of p-IRAK1 and p-IκB-α in HPDLCs stimulated by lipopolysaccharide[J];Shanghai Journal of Stomatology;2007-06
3 Yan Jie,Fang Ping-chu(Dapartment of Microbology, Zhejiang Medical Unviersity ,Hangzhou, 310006);RESEARCH ON BIOLOGICAL ACTIVITY OF THE LIPOPOLYSACCHARIDE OF HELICOBACTER PYLORI[J];CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY;1994-03
【Co-citations】
Chinese Journal Full-text Database 6 Hits
1 SHENG Liping, ZHAO Shifang, JIANG ShuzhengZhejiang University Medical College Affiliated Shao Yi Fu Hospital ,310016;Effects of Porphyromonas Gingivalis Lipopolysaccharide on Fibroblast Attaching to the Surface of the Pure Titanium[J];CHINESE JOURNAL OF ORAL IMPLANTOLOGY;2000-03
2 ZHANG Lei, XU Zi-qing, TANG Guo-hua. (Departmentof Orthodontics, College of Stomatology,Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China);Biological approaches to modification of craniofacial sutures[J];Shanghai Journal of Stomatology;2009-02
3 Sheng-Guo Zhao, Jia-Qi Wang, Guang-Lei Liu, Jin-Bo Cheng, Chun-Gang Zhang, State Key Laboratory of Ani- mal Nutrition, Institute of Animal Science, Chinese Acad- emy of Agriculture Sciences, Beijing 100094, China;Culture of Helicobacter pylori and purification of antigen protein[J];World Chinese Journal of Digestology;2008-10
4 WT5BZ]Li Guijie,Liu Sidang,Zhu Ruiliang,Zhang Shaoxue (College of Animal Science and Technology,Shandong Agricultural University,271018);RESEARCH ON PATHOGENICITY OF THE ENDOTOXIN OF BORDETELLA AVIUM[J];CHINESE JOURNAL OF ANIMAL AND VETERINARY SCIENCES;2000-04
5 Fang Pingchu ;Yan Jie (Department of Microbiology ,Zhejiang , Medical University, Hangzhou, 310006);Invasion and Damage to Mouse Fibroblasts and Cells of Human Gastric Mucosa by the Endotoxin of Helicobacter Pylori[J];CHINESE JOURNAL OF ZOONOSES;1996-02
6 Yan Jie; Fang Pingchu;Huang Lisen (Department of Microbiology, Zhe jiang Medical University, Hangzhou, 310006);Research on Chemical Compositions and SDS-PAGE Characteristics of the Lipopolysaccharide of Helicobacter pylori[J];CHINESE JOURNAL OF ZOONOSES;1996-03
【Secondary Citations】
Chinese Journal Full-text Database 8 Hits
1 Tan Hong,Tang Yal- ing,Zhou Xuedong,Xiao Xiaorong,L i Jiyao. West China School of Stomatology,Sichuan University,Chengdu 610 0 41,China;Effect of Radix Scutellariae on the Growth and form of Porphyromanus endodontics in vitro[J];Journal of Sichuan University (Medical Sciences Edition);2003-03
2 QIN Ke,QIU Li-hong, ZHONG Ming,WANG Zhao-yuan.( College of Stomatology, China Medical University.Shenyang 110002, Liaoning Province, China);The effect of static magnetic field on bone morphogenetic protein-2 in periodontal membrane of the rat[J];Shanghai Journal of Stomatology;2004-04
3 LIN Yuan, WU Bu-ling, SHA Xin-jia, et al (College of Stomatology,The Fourth Military Medical University, Xi′an 710032, China);Influence of LPS on the expression of tissue inhibitor of metalloproteinases in HDPF in vitro[J];Chinese Journal of Conservative Dentistry;2003-02
4 GUO Xi-min, NIU Zhong-ying, XIAO Ming-zhen, et al School of Stomatology, The Fourth Military Medical University,Xi'an,710032$$$$;Detection of Interleukin-8 in exudates from normal and inflamed human dental pulp tissues[J];CHINESE JOURNAL OF CONSERVATIVE DENTISTRY;2000-03
5 Fang Pingchu, Lan Maogong, et al Department of Microbiology Yao Biguang, Huang Huade, et al Medical Department of the First Affiliated Hospital;STUDY ON THE DETECTION OF IgG ANTIBODY AGAINST CAMPYLOBACTER PYLORI BY ELISA AND ITS CLINICAL SIGNIFICANCE[J];Journal of Zhejiang University;1991-06
6 SHI Jun-nan, TANG Rong-yin, WEN Ling-ying, et al(College of Stomatology,The Fourth Military Medical University,Xi′an 710032,China);A 25-year-review on our research progress in pulp biology[J];Chinese Journal of Conservative Dentistry;2005-01
7 Zhang Chunmei,et al.(Facuety of Stomatology,The Copital Institute of Medicine,Beijing 100050);OBSERVATION ON THE ULTRASTRUCTURE OF BLACK PIGMENTED BACTEROIDES[J];CHINESE JOURNAL OF MICROECOLOGY;1996-06
8 ZHANG Feng-qiu, WU Zhi -fen, WAN Ling, et al (College of Stomatology, The Fourth Military Medical University ,Xi' an 710032, China);Production of IL - 6 and TNF - α in PDLC stimulated by lipopolysaccharides[J];Chinese Journal of Conservative Dentistry;2002-10
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved