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《Shanghai Journal of Stomatology》 2009-03
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Identification of cell apoptosis during premaxillary suture expansion in rat

ZHANG Lei,CHEN Rong-Jing,TANG Guo-Hua.(Department of Orthodontics,College of Stomatology,Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai Key Laboratory of Stomatology.Shanghai 200011,China)  
PURPOSE:To identify the incidence of cell apoptosis during premaxillary suture expansion.METHODS:Forty SD rats were randomly allotted into experimental and control groups.The experimental animals were subjected to premaxillary suture expansion by a helix spring with(200±10)g initial force.They were sacrificed on day 1,2,3 and 5(n=5).The sequence of histological changes and osteoblast proliferation in the premaxillary suture were evaluated by hematoxylin and eosin(HE) and toluidine blue staining.Cellular apoptosis was identified by ssDNA immunostaining.The difference between experimental and control groups was analyzed by Student's t test with SPSS 15.0 software package.RESULTS:The width of premaxillary suture was enlarged 1 day after expansion and osteoblasts were accumulated along the suture.After 2 days of stretching,the number of the osteoblasts increased to the peak.New bone deposition was identified on day 3 and projection of suture with bone trabeculae was apparent on day 5.ssDNA immunostaining showed few apoptotic cells in premaxillary suture during natural growth.Upon expansion,cell apoptosis was identified in osteoblasts along the suture and the osteocytes embedded in premaxillary bone,especially on day 2 and day 3(P0.01).CONCLUSION:Apoptotic cell death might regulate the growth modification of premaxillary suture upon mechanical stretching.
【Fund】: 国家自然科学基金(30500572);; 上海市科学技术委员会资助项目(08DZ2271100);; 上海市重点学科建设项目(S30206)~~
【CateGory Index】: R783
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【Citations】
Chinese Journal Full-text Database 3 Hits
1 He Wendan, Lin Wei, Kong Weidong Dept. of stomatology, Overseas Chinese Hospital, Guangzhou;Apoptosis during tensile force application to the interparietal suture of Sprague-Dawley rat[J];Chinese Journal of Orthodontics;2001-04
2 SI Xiao hui 1,JIN Yan 2,YANG Lian jia 2.(1. Research Institute of Stomatology, Ninth People's Hospital, Shanghai Second Medical University, Shanghai, 200011;2.Department of Oral Pathology, Stomatological College, Fourth Military Medical University,;The Effects of Apoptosis during Fracture Healing[J];Shanghai Journal of Stomatology;2001-02
3 Zhang Yunfei,Duan Yinzhong,Jin Yan,et al.Department of Orthodontics,Stomatological Hospital,Fourth Military Medical University,Xi'an 710032;Apoptosis and the expression of Bcl 2 and Bax during orthodontic alveolar bone remodeling in rat[J];JOURNAL OF PRACTICAL STOMATOLOGY;1999-02
【Co-citations】
Chinese Journal Full-text Database 5 Hits
1 He Wendan, Lin Wei, Kong Weidong Dept. of stomatology, Overseas Chinese Hospital, Guangzhou;Apoptosis during tensile force application to the interparietal suture of Sprague-Dawley rat[J];Chinese Journal of Orthodontics;2001-04
2 WU Yun-xia,WANG Lin,ZHANG Wei-bing,MA Jun-qing,WU Rong-ning,ZHANG Wen-jian.(The Research Institute of Stomatology,Department of Orthodontics,School of Stomatology,Nanjing Medical University,Nanjing 210029,China);Detection of apoptosis cells in palatal suture of rat after maxillary expansion[J];Stomatology;2007-04
3 LIU Jin-song1,MA Jian-feng1,WANG Qi2,ZHANG Da-feng1,LIU Chuan-tong1,LIANG Xing2.(1.School and Hospital of Stomatology,Wenzhou Medical College.Wenzhou 325027,Zhejiang Province;2.Department of Prosthodontics,West China College of Stomatology,Sichuan University.Chengdu 610041,Sichuan Province,China);Effects of fluid shear stress on apoptosis of osteoblasts and expression of bcl-2,Bax and caspase-3[J];Shanghai Journal of Stomatology;2008-05
4 LI Xuan,TANG Guo-hua.(Department of Orthodontics,School of Stomatology;Ninth People's Hospital,School of Medicine,Shanghai Jiao Tong University;Shanghai Research Institute of Stomatology.Shanghai 200011,China);Osteoblast apoptosis and growth modification of craniofacial suture[J];China Journal of Oral and Maxillofacial Surgery;2006-03
5 LI Li1,LIU Duo2(1.Guangdong Key Laboratory for Research and Development of Natural Drugs,Guangdong Medical College,Zhanjiang,Guangdong 524023,P.R.China;2.Xinhua Hospital of Dalian University,Dalian,Liaoning 116021,P.R.China);Study on ssDNA labeling index in oral squamous cell carcinoma[J];China Journal of Modern Medicine;2008-24
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 Zhang Yunfei,Duan Yinzhong,Jin Yan,et al.Department of Orthodontics,Stomatological Hospital,Fourth Military Medical University,Xi'an 710032;Apoptosis and the expression of Bcl 2 and Bax during orthodontic alveolar bone remodeling in rat[J];JOURNAL OF PRACTICAL STOMATOLOGY;1999-02
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