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《Acta Agriculturae Shanghai》 1992-02
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SOMATIC EMBRYOGENESIS AND PLANTLETS FROM IMMATURE ZYGOTIC EMBRYOS OF GRAPEVINE

Li Shicheng (Horticultural Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106 ) Shousaku Horiuchi,Ryosuke Mochioka (College of Agriculture, University of Osaka Prefecture, Sakai Osaka Japan 591 )  
Calli and somatic embryos were produced from cultured immature zygotic embryos of Vitis vinifera × V. labrusca hybrid grape eultivars 'Campbell Early' , 'Kyoho' (tetraploid grape), and of V. vinifera 'Koushu' . Immature zygotic explants of these cultivars were collected 47—49 days after anthesis and cultured on NN(Nitsch and Nitsch 1969) and MS(Murashige and Skoog 1962) medium supplemented with 2,4-D and BA 0.5—2.0 mg/l in combination. The frequency of calli formation of explants was 75%—85.7%. After 6 weeks the culli were transferred to NN and MS media containing BA 0.5 mg/l and NAA 0. 03mg/l for somatic embryogenesis. The frequency of somatic embryogenesis produced on NN was 47.6% in 'Campbell Early' grape. It was significantly higher than on MS medium. The Percentage of somatic embryo germination was about 62.5% after isolation from their parental cultures and 28.90% of embryos was developed into normal plantlets when those embryos were transferred into 1/2MS medium without any growth regulators. Seedlings were established in the pots. According to this study an embryo culture method by inducing somatic embryos was discussed for breeding of grapevine.
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