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《ACTA AGRICUL TURAE SHANGHAI》 1997-04
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INDUCTION OF VEGETABLE TETRAPLOID VIA TISSUE CULTURE

Zhang Jianjun; Yin Liqing; Fan Kunhua;Chen Quanqing; Zhang Zhiqi; Zhong Wei jin (Crop Breeding and Cultivation Research Institute,Shanghai Academy of Agricultural Sciences, Shanghai 201106)  
The results of inducing vegetable tetraploid via tissue culture with treatment of colchicine were reported in the paper. The results showed that the method treating plantlets with 3~4 leaves by the solid media supplemented with colchicine, then inducing shoots from them,was the most effective in all chromosome doubling methods,the efficien-cies of chromosome doubling by the method to 4 donor varieties,Shicai,Piaoercai,Guangdong Bolishengcai and Shanyang No. 1, were 13. 33%, 6. 52 %, 15 %and 2. 94 %, respectively. The sensitivities of different genotypes to colchicine were different,treating for 1O~14 d with 100 mg/L colchicine seem to be appropriate for Shicai,whereas treating for 10 d with 50 mg/L colchicine would be appropriate for Guangdong Bolishengcai. The diploid and tetraploid shoots derived from the plantlets treated were easily induced in the meantime, but the chimera was not found in them.
【Fund】: 上海市科学技术发展基金
【CateGory Index】: S636.2
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