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《ACTA AGRICUL TURAE SHANGHAI》 1998-03
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ESTABLISHMENT OF HIGH-FREQUENCY REGENERATION SYSTEM OF LEAF LETTUCE(Lactuca sativa var.Capitata L.)

Sun Yuefang; Huang Jianhua; Lu Ruijn; Zhou Runmei; Wang Yifei(Agro-Biotech Research Center,Shanghai Academy of Agricultural Sciences,Shanghai 201106)Li Haiqian(Honghe District Institute of Agricultural Sciences,Mengzi Yunnan 661100)  
Effects of different hormone combinations, AgNO3 concentrations in media and explants on induction of adventitious buds were compared using 4 leaf lettuce cultivars.The results showed that mean induction rate for the combination of ZT 1 mg/L with IAA 1mg/L was superior to that for the combination of 6--BA0. 5 mg/L with NAA 0.1 mg/L,the rates were 20. 14% and 3. 75%,respectively. The media supplemented with AgNO3 5 mg/L were beneficial to induce more adventitious buds and could increase the rate of induced adventitious buds by 44. 90%. Among the 4 sorts of explants (cotyledon,cotyledon petiole,leaf and leaf petiole), leaf petiole possessed the highest capability for plant regeneration, the rate of induced adventitious buds was over 98% when media were supplemented with AgNO35 mg/L and CH 1000 mg/L.
【Fund】: 上海市农业科学院发展基金
【CateGory Index】: S636
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