OVULE CULTURE TO OBTAIN TRIPLOID PROGENY FROM CROSSES BETWEEN SEEDLESS CULTIVARS AND TETRAPLOID GRAPES
Li Shicheng; Jin Peifang; Jiang Aili Luo Jun (Horticultural Research Institute,Shanghai Academy of Agricultural Sciences, Shanghai 201106 )
The diploid seedless grapes‘Himrod’and‘Canadice’(Vitis vinifera ×V. labrusca )were crossed with a tetraploid pollen parent‘Kyoho’(V. vinifera × V. labrusce)and the hybrid triploid plants were obtained by means of in vitro ovule culture. The rates of surviving embryos and regenerated plantlets were 3.0％ ~ 8.3％ and 0.5％~ 5.0％, respectively. Root tip cells of plantlets derived from diploid ×tetraploid grapes had 57 chromosomes. Ovule culture-derived plants have been established for progeny test in vineyard. Five plants produced flowers in 1995, but all of them were unfruitful. Well fruitful grape clusters were obtained by treating inflorescences with 25 mg/L gibberellic acid solution at full bloom-stage and postflowering stage. Ovule culture of seedless grape can be considered as a new useful way for triploid grape breeding.
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.