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《ACTA AGRICUL TURAE SHANGHAI》 1998-04
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OVULE CULTURE TO OBTAIN TRIPLOID PROGENY FROM CROSSES BETWEEN SEEDLESS CULTIVARS AND TETRAPLOID GRAPES

Li Shicheng; Jin Peifang; Jiang Aili Luo Jun (Horticultural Research Institute,Shanghai Academy of Agricultural Sciences, Shanghai 201106 )  
The diploid seedless grapes‘Himrod’and‘Canadice’(Vitis vinifera ×V. labrusca )were crossed with a tetraploid pollen parent‘Kyoho’(V. vinifera × V. labrusce)and the hybrid triploid plants were obtained by means of in vitro ovule culture. The rates of surviving embryos and regenerated plantlets were 3.0% ~ 8.3% and 0.5%~ 5.0%, respectively. Root tip cells of plantlets derived from diploid ×tetraploid grapes had 57 chromosomes. Ovule culture-derived plants have been established for progeny test in vineyard. Five plants produced flowers in 1995, but all of them were unfruitful. Well fruitful grape clusters were obtained by treating inflorescences with 25 mg/L gibberellic acid solution at full bloom-stage and postflowering stage. Ovule culture of seedless grape can be considered as a new useful way for triploid grape breeding.
【Fund】: 上海市科委局管科技发展基金
【CateGory Index】: S663
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【Co-references】
Chinese Journal Full-text Database 10 Hits
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8 HAN Qing fang 1,XU Ling fei 1,MA Feng wang 1,WANG Zhe zhi 2,REN Xiao lin 1(1 Northwest Sci Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China;2 Collge of Life Science,Shaanxi Normal University,Xian,Shaanxi 710062,China);Adventitious bud regeneration from petiole of pear(Pyrus)[J];Acta Botanica Boreali-occidentalia Sinica;2002-06
9 YAN Shou-wei1,ZHANG Su-li1,ZHANG Guo-jun2,XU Hai-ying2,HU Jian-fang1*(1 College of Agronomy and Biotechnology,China Agricultural University,Beijing 100094,China;2 Forestry and Pomology Institute,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100093,China);Development of Stigma and Style and Formation of Seed with Four Grapevine Cultivars[J];Acta Botanica Boreali-Occidentalia Sinica;2007-03
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