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《Acta Agriculturae Shanghai》 2006-03
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Isolation and identification of porcine circovirus type 2

PAN Rao-lin,SHAN Song-hua,LIU Hong-yun,LI Chun-hua,CHEN Xiao-qing,ZHAO Ben-jin,PENG Li-ying,LIU Sheng,WEN Yi,ZHANG Hua-di,XU Ping,QIAN Zhong-hui(Shanghai Jiamu Biological Products Co.,Ltd,Shanghai 201106,China)  
The expected PCV 2 DNA fragments were amplified by PCR method from the lymph node and spleen tissues derived from suspicious pigs naturally affected with postweaning multisystemic wasting syndrome(PMWS) and the PCR products were sequenced.The sequence analysis showed that the homology was about 98% between the PCR products and standard PCV 2 strains,which indicated that the amplification products had a good specificity and the isolated virus was PCV 2.The detection of five pig farms in Shanghai,Jiangsu,Shandong and Zhejiang provinces confirmed the existence of PCV 2.
【Fund】: 上海市科委重点攻关项目(043919307)
【CateGory Index】: S852.65
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