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《Acta Agriculturae Shanghai》 2008-02
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PCR-DGGE identification of endophytes in morels

SHEN Hong1,2,CHEN Ming-jie1*,ZHAO Yong-chang3,PAN Ying-jie4 (1Microbiology Department,Nanjing Agricultural University,Nanjing 201195,China;2Shanghai Edible Fungi Biotechnology and Engineering Research Center,Shanghai Key Laboratory of Agricultural Genetics and Breeding;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 20110,China;3Institute of Biological Technologies,Yunnan Agricultural Academy,Kunming 650205,China;4Shanghai Fisheries University,Shanghai 200090,China)  
Culturing morels needs soil sealing which could contain beneficial microorganism to stimulate the growth of morels.In order to know about the bacterial population closely related with the growth of morels,the bacteria contained in the fruiting body of morels were identified by means of a PCR-DGGE technology instead of pure culture methods.The sequencing found a total of 7 kinds of bacteria,and to a certain extent,the method of PCR-DGGE could reflect the bacterial community structure in morels.
【Fund】: 上海市科委基金项目(06DZ22036);; 农业部公益性行业科研专项(nyhyzx07-008)资助
【CateGory Index】: S646
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