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《Acta Agriculturae Shanghai》 2010-03
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Determination of D-calcium pantothenate and D-biotin in premixed feeds by ultrahigh-performance liquid chromatography

LEI Ping (Institute for Agro-Product Quality Standards and Testing Technology,SAAS,Shanghai 201106,China)  
Using ultrahigh-performance liquid chromatography(UPLC) could jointly determine Dcalcium pantothenate and D-biotin levels in premixed feeds with an ACQUITY UPLC BEH C18 as a chromatographic column,methanol and trifluoroacetic acid as a mobile phase,and constant gradient elution. The above two components were completely separated within 4 minutes,and each component's concentration presented a good linear relationship with the peak area in the range of 5~200 mg/mL. The detection limit(S/N = 3) was 1.5μg/mL for D-calcium pantothenate and 1.5μg/mL for D-biotin.
【CateGory Index】: S816.17
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