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《Shanghai J.of Immunology》 2002-06
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Study on the Tumorigenicity of Nasopharyngeal Carcinoma Cells Transfected with Human IL-18 cDNA in Hu-PBL-SCID Mice

TAO Jian-ping 1,LI Man-zhi 2,WANG Hui-min 2,GUO Xiang 3,HONG Ming-huang 3(1 Department of Microbiology,Sun Yat-Sen University of Medical Scienc e,Guangzhou 510080;2 Cancer Institute;3 Cancer Hospital)  
To study the tumorigenicity of nasophary ngeal carcinoma (NPC) cells in the hu-PBL-SCID mouse model,human IL-18 cDNA had been amplified from PBMC through RT-PCR,cloned and then subcloned into eukaryotic expression vector pcDNA3 at EcoR I and BamH I si tes and transfected into a NPC-derived cell-line SUNE cells The transfected and non-transfected SUNE cells were inoculated subcutaneously in the right flan k of hu-PBL-SCID mice. Results showed that the recombinant eukaryotic expressi on vector could highly express the hu-IL-18 in SUNE cells but no hu-IL-18 ex pression in the control cells The concentration of hu-IL-18 in the supernata nts of the transfected SUNE cell cultures as detected by ELISA was (85±10)pg/ml but that in the supernatants of non-transfected cells was less than 5 pg/ml After inoculation to the hu-PBL-SCID mice,the pcDNA3/hu-IL-18 trans fected SUNE cells grew slowly than the non-transfected cells The tumors were excised 50 days later and weighed,and it was found by statistical analysis that the size and mean weight of the tumors from the former group of mice were signif icantly lower in contrast to those of the latter These results indicate that t he tumorigenicity of nasopharyngeal carcinoma cells could be effectively weaken by the expression of IL-18 in vivo -
【CateGory Index】: R739.63
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