Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Current Immunology》 2009-03
Add to Favorite Get Latest Update

Effect on Tween 80 on the degranulation of RBL-2H3 cells

ZHANG Jia1,2,LI Peng2,LI Yi-kui2,LI Lian-da2(1.School of Basic Medical Science,Beijing University of Chinese Medicine,Beijing 100029,China;2.Experimental Research Center of Xiyuan HosPital,China Academy of Traditional Chinese Medicines,Beijing 100091,China)  
To investigate the effect of Tween 80 on the mast cell degranulation in RBL-2H3 cells and to find out the possible mechanism of the anaphylactoid reaction induced by Tween 80,different concentrations of Tween 80 solution were incubated with RBL-2H3 cells.The releasing rate of the anaphylaxis activating substances,such as histamine and β-P-nitrophyenyl-N-acetyl-β-D-glucosaminide,were detected by ELISA and biochemical method and the translocation of phosphatidylserine(PS)in cell membrane was assayed by Annexin-V-FITC fluorescent antibody staining(flow cytometry and fluorescent microscopy).It was demonstrated that the releasing rates of these anaphylaxis activating substances were increased after co-incubation of the cells with 250,1 250 and 6 250 mg/L concentrations of Tween 80 in a dose-dependent manner(P0.01 or 0.05).Meanwhile,the releasing rate of PS translocation in cell membrane after treatment with Tween 80 increased also in a dose-dependent manner(P0.01).However,the degranulation induced by Tween 80 independent on the extracellular calcium;no difference could be detected upon addition of calcium solution or without addition(P0.05).From these observations,it is concluded that Tween 80 can induce degranulation of mast cells directly to release active mediators of anaphylaxis and this may be one of the cause of the anaphylactoid reaction induced by Tween 80.
【Fund】: 中药注射剂的安全性评价研究 国家十一五科技支撑计划资助(2006BAI14B05)
【CateGory Index】: R285
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 5 Hits
1 Feng Wenyu,et al Pharmacy Research Institute,Luzhou Medical College;RESEARCH ON TOXICITY OF CHINESE DRUG INJECTION CONTAINING POLYSORBATE 80 FTO DIFFERENT ANIMALS[J];Journal of Luzhou Medical College;2007-02
2 ZHANG Jia1,2,LI Yi-kui1,LI Lian-da1,HAO Wei1,YAN Wei-juan1 (1 Experimental Research Center of Xiyuan Hospital,China Academy of Traditional Chinese Medicines,Beijing 100091,China;2 School of Basic Medical Science,Beijing University of Chinese Medicine,Beijing 100029,China);Sensitization of Beagle dogs to Houttuynia cordata distillation mixed with three solubilizers[J];Chinese Journal of New Drugs;2008-17
3 LU Yun-Bi, JIANG Bo, ZHOU Han-Liang (Department of Pharmacology, School of Medical Science, Zhejiang University, Hangzhou 310031, China);Effects of salbutamol and sodium cromoglicate on phospholipase D activity during the degranulation of rat peritoneal mast cell[J];Chinese Journal of Pharmacology and Toxicology;2001-05
4 YUAN Li, GUO Wei 1,LIU Shui-Ping 1 Department of Biochemistry and Molecular Biology, Nanjing Medical University, Na njing 210029, Jiangsu, China; 1 Department of Forensic Science, Sun yat-sen University, Guangzhou 510080, Guangdong, China;Inhibition of dexamethasone on degranulation of mast cells and its mechanism[J];Chinese Journal of Clinical Pharmacology and Therapeutics;2003-06
5 Wu Yi1,2, Jin Shaohong1 (1National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050; 2School of Pharmacy China Pharmaceutical University);Progress of Pharmacological Properties,Pharmacokinetics Properties and Analytic Method of Pharmaceutical Adjuvant Tween 80[J];Chinese Pharmaceutical Affairs;2008-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LUO Gang-jian,GAN Xiao-liang,SHEN Ning,HEI Zi-qing,LI Shang-rong,CHEN Li-xin Department of Anesthesiology,Third Affiliated Hospital,SUN Yat-sen University,Guangzhou 510630,China;Cromolyn sodium ameliorates rat left cardiac function during intestinal ischemia-reperfusion[J];Journal of Southern Medical University;2007-05
2 HEI Zi-qing~(1,*),GAN Xiao-liang~1,LI Shang-rong~1,et al Department of Anesthesiology,3 rd Affiliated Hospital,SUN Yat-sen University,Guangzhou 510630,China;Cromolyn sodium protect lung injury caused by intestinal ischemia reperfusion in rats[J];International Journal of Anesthesiology and Resuscitation;2006-06
3 SHEN Ning,GAN Xiao-liang,PANG Hu-yu,HE Zi-qing Department of Anesthesiology,Third Affiliated Hospital of Sun Yat-sen Uviversity,Guangzhou 510630,China;Role of sodium cromoglycate in brain protection and its effects on tumor necrosis factor-α and interleukin-1β after global cerebral ischemia-reperfusion injury in gerbils[J];Journal of Southern Medical University;2009-03
4 DU Xiao-fen1, XIE Bi-jun1, YANG Er-ning1, SUN Zhi-da1, ZHANG Ling-zhen2, ZHONG Ren-qian2 1.Department of Natural Plant, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, P.R.China 2.Department of Clinical Laboratory, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R.China;Procyanidins from lotus seedpod and their cytotoxity against cancer cell lines as well as normal cells[J];Journal of Qilu Oncology;2004-11
5 ZHANG Jia1,2,LI Yi-kui1,LI Lian-da1,ZHAO Le1 (1 Experimental Research Center of Xiyuan Hospital,China Academy of Traditional Chinese Medicines,Beijing 100091,China;2 School of Basic Medical Science,Beijing University of Chinese Medicine,Beijing 100029,China);Acute toxicity of Tween-80,Poloxamer188 and other two solubilizers in mice[J];Chinese Journal of New Drugs;2008-17
6 ZHANG Jia1,2,LI Yi-kui1,LI Lian-da1,HAO Wei1,YAN Wei-juan1 (1 Experimental Research Center of Xiyuan Hospital,China Academy of Traditional Chinese Medicines,Beijing 100091,China;2 School of Basic Medical Science,Beijing University of Chinese Medicine,Beijing 100029,China);Sensitization of Beagle dogs to Houttuynia cordata distillation mixed with three solubilizers[J];Chinese Journal of New Drugs;2008-17
7 GAN Xiao-liang,HEI Zi-qing,LUO Gang-jian,LI Shang-rong,CHEN Li-xin,CAI Jun(Department of Anesthesiology,The Third Affiliated Hospital,Sun Yat-sen University,Guangzhou 510630,China.);Effects of mast cell membrane stabilizer on histamine concentration,MDA content and SOD activity in small intestine after ischemia-reper-fusion injury in rats[J];Chinese Journal of Pathophysiology;2008-01
8 Liu Yanmei1,Pu Xufeng2,Lei Lei1,Zhang Debo2,Zhang Liang2,Peng Hengyang1(2 Chengdu Center of Food and Drug Control,Chengdu 610045;1 Chengdu University of TCM,Chengdu 610072);Experimental study on adverse reaction by Intravenous Yuxingcao injection Ⅱ. Allergy research for guinea pigs[J];Pharmacology and Clinics of Chinese Materia Medica;2008-02
9 GUO Yu,YANG Qing-min,WANG Jing-yi,QI Lin,GONG Xin-jiang(Division of Research and Development,Qilu Pharmaceutical Co.,LTD.,Shandong Jinan 250100,China);Study on RBL-2H3 mast cell degranulation caused by tween-80[J];Chinese Journal of Hospital Pharmacy;2008-19
10 Wu Yi1,2, Jin Shaohong1 (1National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050; 2School of Pharmacy China Pharmaceutical University);Progress of Pharmacological Properties,Pharmacokinetics Properties and Analytic Method of Pharmaceutical Adjuvant Tween 80[J];Chinese Pharmaceutical Affairs;2008-08
【Secondary Citations】
Chinese Journal Full-text Database 3 Hits
1 GUO Wei,CHEN Yu-chuan,LIU Shui-ping,WANG Shu-guang, KONG Qing-yu(Department of Forensic Pathology,Sun Yat-sen University of Medical Science,Guangzhou 510080);A New Method for Quantitative Testing of Degranulation in Peritoneal Mast Cells[J];Shanghai J.of Immunology;2002-01
2 LI Ping, LU YunBi, ZHOU HanLiang, CHEN JiQiang (Dept of Pharmacology, Medical School, Zhejiang University, Hangzhou 310031);Changes of phospholipase D activity of exudate leukocytes in rat carrageenan-induced pleurisy[J];CHINESE PHARMACOLOGICAL BULLETIN;2000-01
3 Han Zhaohong, Liang Yuedong Beijing Hospital of Gynecology and Obstetrics, Beijing 100006, P.R.China;An Analysis of Adverse Reactions to Hertleaf Houttuynia Herb Injection in 155 Cases[J];Chinese Journal of Pharmaco Epidemiology;2004-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved