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《Journal of Shanghai Jiaotong University(Agricultural Science)》 2009-06
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A Conductometric Sensor for Determination of HS-CAP in Milk Based on Molecularly Imprinted Films

LI Feng,WANG Li,LIU Guo-yan,CHAI Chun-yan (School of Agricultrue & Biology,Shanghai Jiaotong University,Shanghai 200240,China)  
Molecularly imprinted films(MIF) with specific recognition sites for Chloramphenicol succinate(HS-CAP) were prepared on screen printed electrodes by photopolymerisation.The superficial characteristic of the MIF was investigated using scanning electron microscope(SEM).A conductometric sensor system for detecting HS-CAP was constructed by incorporating the screen printed electrodes covered with MIF into electrode slots of a conductometer.Calibration curve for HS-CAP was established and determination of HS-CAP in milk samples was conducted,using the conductometric sensor system.The sensors based on MIF showed high selectivity to HS-CAP and the response of MIF-sensors to concentration of HS-CAP displayed a linear correlation over a range from 0.2 mg·L~(-1) to 1.0 mg·L~(-1) with a detection limit of 0.05 mg·L~(-1).The recoveries of 94.3%~98.5%were obtained for HS-CAP in milk samples.
【Fund】: 国家“863”计划项目(2006AA10Z439);; 国家自然基金项目(N030571368)
【CateGory Index】: TP274.4
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